中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2010年
4期
295-299
,共5页
刘德凤%赖凌云%陈靖%顾勇%蔡晖
劉德鳳%賴凌雲%陳靖%顧勇%蔡暉
류덕봉%뢰릉운%진정%고용%채휘
醛固酮%氯化钠%膳食%螺内酯%上皮钠通道%WNK4
醛固酮%氯化鈉%膳食%螺內酯%上皮鈉通道%WNK4
철고동%록화납%선식%라내지%상피납통도%WNK4
Aldosterone%Sodium chloride,dietary%Spironolactone%Epithelial sodium channel%WNK4
目的 通过建立生理条件下的盐负荷饮食大鼠模型,观察醛同酮和WNK4在水盐代谢调节中的作用.方法 将SD大鼠分为5组:高盐组(H,4%NaCl)、正常盐组(N,0.4%NaCl)、低盐组(L,0.07%NaCl)、高盐加醛固酮组(H+A,4%NaCl+1 mg·kg~(-1)·d~(-1)醛固酮)、低盐加螺内酯(L+S,0.07%NaCl+0.1 g·kg~(-1)·d~(-1)螺内酯),所有大鼠自由饮水,喂养2周.用放射免疫法检测血浆醛同酮的变化.应用实时定量PCR和Western印迹法检测大鼠肾脏上皮钠通道γ亚基(γENaC)、WNK4的mRNA和蛋白的变化.结果 H组大鼠血浆醛同酮水平低于N组(P<0.05),H+A组高于H组(P<0.05);L组大鼠血浆醛固酮水平高于N组(P<0.05),显示SD大鼠造模成功.L组大鼠肾脏γENaC蛋白表达高于N组,但是L+S低于L组;同时H组低于N组,H+A组高于H组,差异均有统计学意义(P<0.05).mRNA变化趋势和蛋白变化趋势一致.H组肾脏WNK4的蛋白表达高于N组,但是H+A组低于H组;同时L组低于N组,L+S组高于L组,差异均有统计学意义(P<0.05).mRNA的变化趋势和蛋白的变化趋势一致.结论 饮食中的盐可以调节γENaC在肾脏的蛋白表达,醛固酮和WNK4都参与了机体对盐的调节,WNK4受到醛同酮的负调节作用.
目的 通過建立生理條件下的鹽負荷飲食大鼠模型,觀察醛同酮和WNK4在水鹽代謝調節中的作用.方法 將SD大鼠分為5組:高鹽組(H,4%NaCl)、正常鹽組(N,0.4%NaCl)、低鹽組(L,0.07%NaCl)、高鹽加醛固酮組(H+A,4%NaCl+1 mg·kg~(-1)·d~(-1)醛固酮)、低鹽加螺內酯(L+S,0.07%NaCl+0.1 g·kg~(-1)·d~(-1)螺內酯),所有大鼠自由飲水,餵養2週.用放射免疫法檢測血漿醛同酮的變化.應用實時定量PCR和Western印跡法檢測大鼠腎髒上皮鈉通道γ亞基(γENaC)、WNK4的mRNA和蛋白的變化.結果 H組大鼠血漿醛同酮水平低于N組(P<0.05),H+A組高于H組(P<0.05);L組大鼠血漿醛固酮水平高于N組(P<0.05),顯示SD大鼠造模成功.L組大鼠腎髒γENaC蛋白錶達高于N組,但是L+S低于L組;同時H組低于N組,H+A組高于H組,差異均有統計學意義(P<0.05).mRNA變化趨勢和蛋白變化趨勢一緻.H組腎髒WNK4的蛋白錶達高于N組,但是H+A組低于H組;同時L組低于N組,L+S組高于L組,差異均有統計學意義(P<0.05).mRNA的變化趨勢和蛋白的變化趨勢一緻.結論 飲食中的鹽可以調節γENaC在腎髒的蛋白錶達,醛固酮和WNK4都參與瞭機體對鹽的調節,WNK4受到醛同酮的負調節作用.
목적 통과건립생리조건하적염부하음식대서모형,관찰철동동화WNK4재수염대사조절중적작용.방법 장SD대서분위5조:고염조(H,4%NaCl)、정상염조(N,0.4%NaCl)、저염조(L,0.07%NaCl)、고염가철고동조(H+A,4%NaCl+1 mg·kg~(-1)·d~(-1)철고동)、저염가라내지(L+S,0.07%NaCl+0.1 g·kg~(-1)·d~(-1)라내지),소유대서자유음수,위양2주.용방사면역법검측혈장철동동적변화.응용실시정량PCR화Western인적법검측대서신장상피납통도γ아기(γENaC)、WNK4적mRNA화단백적변화.결과 H조대서혈장철동동수평저우N조(P<0.05),H+A조고우H조(P<0.05);L조대서혈장철고동수평고우N조(P<0.05),현시SD대서조모성공.L조대서신장γENaC단백표체고우N조,단시L+S저우L조;동시H조저우N조,H+A조고우H조,차이균유통계학의의(P<0.05).mRNA변화추세화단백변화추세일치.H조신장WNK4적단백표체고우N조,단시H+A조저우H조;동시L조저우N조,L+S조고우L조,차이균유통계학의의(P<0.05).mRNA적변화추세화단백적변화추세일치.결론 음식중적염가이조절γENaC재신장적단백표체,철고동화WNK4도삼여료궤체대염적조절,WNK4수도철동동적부조절작용.
Objective To examine the role of aldosterone and WNK4 in redistribution of epithelial Na(+)channel(γENaC)in response to a high-salt diet.Methods 200-250 g weight Sprague-Dawley rats were randomly assigned to five groups: normal salt diet(N,0.4% NaCl),high salt diet(H,4% NaCl),low salt diet(L,0.07% NaCl),H treated with aldosterone(H+A,4% NaCl with osmotic mini-pump at 1 mg·kg~(-1)·d~(-1) aldosterone)，and L treated with spironolactone(L+S，0．07% NaCl with 0．1 g·kg~(-1)·d~(-1) spironolactone)．Results The high salt diet decreased
γENaC protein and the low salt diet increased γENaC protein.However,the γENaC of H+A group was higher compared with that of H group and the γENaC of L+S group was lower compared with that of L group.Radioimmunoassay demonstrated that the concentration of plasma aldosterone was decreased by high salt diet compared with that by normal salt diet.On the contrary to the aldosterone,the expression of WNK4 was remarkable enhanced in H group and decreased in L group,but it was decreased in H+A group and enhanced in L+S group.Conclusions The decrease of γENaC during high salt diet is regulated by aldosterone and involves WNK4 activity as well.WNK4 kinase is affected negatively by aldosterone in kidney.
