CAJ | 학술논문

目的建立云南苗族巴德-毕氏综合征(Bardet-Biedl syndrome,BBS)家系外周血B淋巴母细胞系,为研究BBS的发病机制、诊断和治疗提供长期的标本来源.方法取1个BBS核心家系成员新鲜肝素抗凝全血,采用EB病毒转化技术,并加入环孢霉素A(cyclosporine A,CyA)抑制T淋巴细胞,将B淋巴细胞转化成永生化淋巴母细胞系.结果成功建立了苗族BBS核心家系中12个个体的永生细胞株.结论 EB病毒转化技术可有效保存原来个体完整的基因组,为以后的相关研究提供了长期的DNA资源.
목적 건립운남묘족파덕-필씨종합정(Bardet-Biedl syndrome,BBS)가계외주혈B림파모세포계,위연구BBS적발병궤제、진단화치료제공장기적표본래원.방법 취1개BBS핵심가계성원신선간소항응전혈,채용EB병독전화기술,병가입배포매소A(cyclosporine A,CyA)억제T림파세포,장B림파세포전화성영생화림파모세포계.결과 성공건립료묘족BBS핵심가계중12개개체적영생세포주.결론 EB병독전화기술가유효보존원래개체완정적기인조,위이후적상관연구제공료장기적DNA자원.
Objective To establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome (BBS), in order to provide a long-term source of material for research. Methods With Epstein-Barr virus transformation of B cells and addition of cyclosporine A to inhibit the activity of T cells, fresh anticoagulated blood samples with heparin were collected from 12 members of the core pedigree,and were used to establish the immortalized lymphoblastoid cell lines of B lymphocytes. Results Twelve immortalized lymphoblastoid cell lines of the core BBS pedigree were obtained successfully. Conclusion The immortalized B lymphoblastoid cell lines of the Miao pedigree with BBS can preserve the whole genome information and provide long-term research materials for BBS study.