CAJ | 학술논문

目的探讨轻度认知功能障碍(MCI)及阿尔茨海默病(AD)患者外周淋巴细胞糖原合成酶激酶-3(GSK-3)的变化.方法用放射性配体结合试验检测正常老年人(对照组)、MCI患者(MCI组)及AD患者(AD组)外周淋巴细胞GSK-3的活性,并用Western blot法检测GSK-3β Ser9位点的磷酸化水平及总GSK-3β表达.结果 AD组及MCI组GSK-3活性分别为(1.32±0.09)和(1.22±0.10),明显高于对照组的(1.00±0.06),差异有统计学意义(均P<0.01);AD组及MCI组GSK-3β Ser9位点的磷酸化水平分别为(0.82±0.07)和(0.87±0.08),明显低于对照组的(1.01±0.07),差异有统计学意义(P<0.05或0.01);AD组及MCI组总GSK-3β表达水平与对照组比较,差异无统计学意义(P>0.05).结论 MCI及AD患者外周淋巴细胞GSK-3的活性升高,可能对此类疾病的早期诊断有潜在的价值.
목적 탐토경도인지공능장애(MCI)급아이자해묵병(AD)환자외주림파세포당원합성매격매-3(GSK-3)적변화.방법 용방사성배체결합시험검측정상노년인(대조조)、MCI환자(MCI조)급AD환자(AD조)외주림파세포GSK-3적활성,병용Western blot법검측GSK-3β Ser9위점적린산화수평급총GSK-3β표체.결과 AD조급MCI조GSK-3활성분별위(1.32±0.09)화(1.22±0.10),명현고우대조조적(1.00±0.06),차이유통계학의의(균P<0.01);AD조급MCI조GSK-3β Ser9위점적린산화수평분별위(0.82±0.07)화(0.87±0.08),명현저우대조조적(1.01±0.07),차이유통계학의의(P<0.05혹0.01);AD조급MCI조총GSK-3β표체수평여대조조비교,차이무통계학의의(P>0.05).결론 MCI급AD환자외주림파세포GSK-3적활성승고,가능대차류질병적조기진단유잠재적개치.
Objective To explore changes in glycogen synthase kinase-3 (GSK-3) levels in the lymphocytes of patients with mild cognition impairment (MCI) and Alzheimer's disease (AD). Methods GSK-3 activity was measured by 32P liquid scintillography in a control group, an MCI group and an AD group. The expressions of GSK-3βat Ser9 and total GSK-3β were determined by western blotting. Results GSK-3 levels were significantly higher in the AD and MCI groups than in the control group. The phosphorylation of GSK-3βat Ser9 was significantly lower in the AD and MCI groups compared with the control group, but there was no difference in the phosphorylation of total GSK-3β among the three groups. Conclusions Higher GSK-3 activity in the lymphocytes might have value in the diagnosis of MCI and early AD.