CAJ | 학술논문

目的证实在非甲～庚型病毒性肝炎患者肝组织中TT病毒(transfusion-transmittedvirus，TTv)的存在。方法采用地高辛素标记TTV DNA探针以原位杂交技术对51例血清学病毒标记非甲～戊型、免疫组化检测肝组织中HBsAg、HCV NS3Ag及HGV NS5Ag阴性的病毒性肝炎患者石蜡包埋肝组织进行了检测。结果各型病毒性肝炎肝组织中TTV基因的总检出率为27.5％，其中急性轻型肝炎的检出率为30.8％(4/13)，急性重型肝炎为1/8，亚急性重型肝炎为3/7，慢性肝炎为2/6，活动性肝硬化为2/9，慢性重型肝炎为1/4，原发性肝癌为1/4。TTV DNA表达于肝细胞核或胞质内，以核型多见。在急性肝炎，TTV阳性细胞弥漫分布于肝小叶内，慢性肝炎于汇管区附近较为密集，而在肝硬化病例，阳性细胞在假小叶内多呈片簇状不规则分布。结论在不明原因病毒性肝炎患者血清及肝组织中TTV DNA的检出表明TTV为一种新型的肝炎病毒，TTV为一种嗜肝性病毒。
목적증실재비갑～경형병독성간염환자간조직중TT병독(transfusion-transmittedvirus，TTv)적존재。방법채용지고신소표기TTV DNA탐침이원위잡교기술대51례혈청학병독표기비갑～무형、면역조화검측간조직중HBsAg、HCV NS3Ag급HGV NS5Ag음성적병독성간염환자석사포매간조직진행료검측。결과각형병독성간염간조직중TTV기인적총검출솔위27.5％，기중급성경형간염적검출솔위30.8％(4/13)，급성중형간염위1/8，아급성중형간염위3/7，만성간염위2/6，활동성간경화위2/9，만성중형간염위1/4，원발성간암위1/4。TTV DNA표체우간세포핵혹포질내，이핵형다견。재급성간염，TTV양성세포미만분포우간소협내，만성간염우회관구부근교위밀집，이재간경화병례，양성세포재가소협내다정편족상불규칙분포。결론재불명원인병독성간염환자혈청급간조직중TTV DNA적검출표명TTV위일충신형적간염병독，TTV위일충기간성병독。
Objective To demonstrate the existence of TT virus (transfusion-transmitted virus, TTV) in liver tissues of patients with unknown etiology. Methods Paraffin-embedded liver tissues from 51 cases who were non A-E hepatitis serologically and immunohistochemically negative for HBsAg, HCV NS3 antigen and HGV NS5 antigen were tested by in situ hybridizationwith Dig TTV DNA probe. Results The total positive rate of TTV DNA was 27.5 96 ( 14/51 ). Among the different pathological types, the positive rates were 30.8% (4/13) in acute mild hepatitis, 12. 596 (1/8) in acute fulminante hepatitis,42.9% (3/7) in subacute fulminante hepatitis,33.3% (2/6) in chronic hepatitis,22.2% (2/9) in active liver cirrhosis,25% (1/4) in chronic fulminante hepatitis and 25% (1/4) in primary liver carcinoma respectively. Hybridization signals were detected within the nuclei or cytoplasm of the hepatocyte and most of them in nuclei. The positive cells were diffusely scattered in the intralobular areas in acute hepatitis and aggregated in periportal areas in chronic hepatitis or in the form of clustering in pseudolobules in active liver cirrhosis. Conclusion Our results suggest that the TTV detected from liver tissues or sera from patients with unknown etiology is a novel virus, which belongs to hepadnavirus.