中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2000年
3期
213-235
,共23页
刘传新%苏生利%黄涛%傅继华%赵世立%邢辉%潘品良%范秀娟%冯毅%邵一鸣
劉傳新%囌生利%黃濤%傅繼華%趙世立%邢輝%潘品良%範秀娟%馮毅%邵一鳴
류전신%소생리%황도%부계화%조세립%형휘%반품량%범수연%풍의%소일명
HIV-1%膜蛋白质类%序列分析%变异(遗传学)%基因
HIV-1%膜蛋白質類%序列分析%變異(遺傳學)%基因
HIV-1%막단백질류%서렬분석%변이(유전학)%기인
HIV- 1%Membrane proteins%Sequence analysis%Variation(genetics)%Gene
目的 了解流行于山东境内HIV-1毒株的亚型分布及变异情况，分析其来源并推测其流行趋势。方法 采集了25份HIV-1抗体阳性感染者的全血分离单核细胞(PBMC)，提取前病毒DNA，经nested-PCR扩增HIV-1膜蛋白(env)基因的C2-V3区并进行序列测定和亚型分析。结果 25份HIV-1阳性感染者的PBMC样品中扩增到24份可用于序列测定的HIV-1env基因片段，经序列测定和基因分析鉴别出共有5种HIV-1M亚群基因亚型：A、B、C、E和B＇亚型。13名有偿献血人员均为B＇(泰国B)亚型，10名回国劳工及1名回国劳工的女性配偶中存在A、B、C、E四种亚型。结论 山东省HIV-1各种亚型毒株感染情况复杂，因此应加强对献血者及回国劳工等高危人群的检测和控制，以防HIV-1各亚型毒株在全省的蔓延。
目的 瞭解流行于山東境內HIV-1毒株的亞型分佈及變異情況，分析其來源併推測其流行趨勢。方法 採集瞭25份HIV-1抗體暘性感染者的全血分離單覈細胞(PBMC)，提取前病毒DNA，經nested-PCR擴增HIV-1膜蛋白(env)基因的C2-V3區併進行序列測定和亞型分析。結果 25份HIV-1暘性感染者的PBMC樣品中擴增到24份可用于序列測定的HIV-1env基因片段，經序列測定和基因分析鑒彆齣共有5種HIV-1M亞群基因亞型：A、B、C、E和B＇亞型。13名有償獻血人員均為B＇(泰國B)亞型，10名迴國勞工及1名迴國勞工的女性配偶中存在A、B、C、E四種亞型。結論 山東省HIV-1各種亞型毒株感染情況複雜，因此應加彊對獻血者及迴國勞工等高危人群的檢測和控製，以防HIV-1各亞型毒株在全省的蔓延。
목적 료해류행우산동경내HIV-1독주적아형분포급변이정황，분석기래원병추측기류행추세。방법 채집료25빈HIV-1항체양성감염자적전혈분리단핵세포(PBMC)，제취전병독DNA，경nested-PCR확증HIV-1막단백(env)기인적C2-V3구병진행서렬측정화아형분석。결과 25빈HIV-1양성감염자적PBMC양품중확증도24빈가용우서렬측정적HIV-1env기인편단，경서렬측정화기인분석감별출공유5충HIV-1M아군기인아형：A、B、C、E화B＇아형。13명유상헌혈인원균위B＇(태국B)아형，10명회국노공급1명회국노공적녀성배우중존재A、B、C、E사충아형。결론 산동성HIV-1각충아형독주감염정황복잡，인차응가강대헌혈자급회국노공등고위인군적검측화공제，이방HIV-1각아형독주재전성적만연。
Objective The aim of this study was to understand the distribution of HIV - 1 strain sub-types and their variation in Shandong Province and to analyze their source and predict their epidemic trend. Method
We collected the whole blood from 25 anti - HIV- 1 antibody positive cases and separated the peripheral bloodmononuclear cells. The provirus DNA was extracted and amplified by nested- PCR, after which the HIV - 1 mem-brane protein gene(env) C2 - V3 region was sequenced and its subtype was analyzed. Results Among 25 HIV -1 antibody positive cases,24 HIV- 1 env gene fragments were amplified and analyzed.5 subtypes of Group HIV-1 M were identified among these cases,that are subtypes A,B,B' ,C and E.13 occupational blood donors were allclassified as subtype B' (Thailand B').A,B,C or E subtypes were discovered in 10 returned laborers workingabroad and 1 female spouse of one laborer. Conclusion The condition of HIV - 1 subtype infection was compli-cared in Shandong Province. So, it is important to strengthen the surviellance and control of HIV - 1 subtype straininfection in high risk populations especially the occupational blood donors and returned laborers working abroad toprevent its spread in Sbandong Province.
