中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2009年
7期
593-596
,共4页
严淑贤%陈国梁%胡跃%徐昱%孙新芬%廖康煌
嚴淑賢%陳國樑%鬍躍%徐昱%孫新芬%廖康煌
엄숙현%진국량%호약%서욱%손신분%료강황
紫外线%皮肤衰老%抗氧化剂%环氮氧化物
紫外線%皮膚衰老%抗氧化劑%環氮氧化物
자외선%피부쇠로%항양화제%배담양화물
Ultraviolet ray%Skin aging%Antioxidants%Cyclic N-oxides
目的 探讨建立豚鼠皮肤的光老化模型及外用氮氧化物Tempol对豚鼠光老化皮肤的保护作用. 方法 以模拟目光光源(SSR)建立豚鼠皮肤的光老化模型,以苏木素一伊红和Weigert染色法及透视电镜观察真皮结构、成纤维细胞和弹力纤维结构的变化.豚鼠在每次照射前外用5mg/ml或0.5 mg/ml的Tempol,探讨Tempol对上述变化的保护作用. 结果 豚鼠以亚红斑量SSR照射17周,光照部位真皮浅层出现了典型的光化性弹力纤维变性损害,成纤维细胞损伤甚至破碎.照射前外用Tempol可以预防上述表现出现,Tempol以5 mg/ml保护作用更强. 结论 利用豚鼠可构建稳定的光老化动物模型,抗氧化剂Tempol对豚鼠皮肤光老化损害有防护作用.
目的 探討建立豚鼠皮膚的光老化模型及外用氮氧化物Tempol對豚鼠光老化皮膚的保護作用. 方法 以模擬目光光源(SSR)建立豚鼠皮膚的光老化模型,以囌木素一伊紅和Weigert染色法及透視電鏡觀察真皮結構、成纖維細胞和彈力纖維結構的變化.豚鼠在每次照射前外用5mg/ml或0.5 mg/ml的Tempol,探討Tempol對上述變化的保護作用. 結果 豚鼠以亞紅斑量SSR照射17週,光照部位真皮淺層齣現瞭典型的光化性彈力纖維變性損害,成纖維細胞損傷甚至破碎.照射前外用Tempol可以預防上述錶現齣現,Tempol以5 mg/ml保護作用更彊. 結論 利用豚鼠可構建穩定的光老化動物模型,抗氧化劑Tempol對豚鼠皮膚光老化損害有防護作用.
목적 탐토건립돈서피부적광노화모형급외용담양화물Tempol대돈서광노화피부적보호작용. 방법 이모의목광광원(SSR)건립돈서피부적광노화모형,이소목소일이홍화Weigert염색법급투시전경관찰진피결구、성섬유세포화탄력섬유결구적변화.돈서재매차조사전외용5mg/ml혹0.5 mg/ml적Tempol,탐토Tempol대상술변화적보호작용. 결과 돈서이아홍반량SSR조사17주,광조부위진피천층출현료전형적광화성탄력섬유변성손해,성섬유세포손상심지파쇄.조사전외용Tempol가이예방상술표현출현,Tempol이5 mg/ml보호작용경강. 결론 이용돈서가구건은정적광노화동물모형,항양화제Tempol대돈서피부광노화손해유방호작용.
Objective To explore the establishment of skin photoaging model and the protective effects of nitroxide tempol on skin in guinea pig. Methods The guinea pig skin photoageing model was established by using solar-simulaten radiation (SSR). Dermal structure was observed with hematoxylin-eosin staining. The structure and expression of elastic fiber were analyzed by Weigert's staining. The uhrastructure of dermal fibroblasts and elastic fiber were observed by electron microscopy. Tempol was used before each exposure at the concentration of 5 mg/ml or 0. 5 mg/ml, and the protective effects of tempol on skin were assessed. Results After seventeen weeks' exposure, there was typical "solar elastosis" damage in the upper dermis. Mature elastic fibers were severely degraded and there was large amount of elastotic material accumulated in the upper dermis. Dermal fibroblasts appeared metabolically hyperactive and mitochondria in the cells were damaged. Some cells even broke up. Tempol at the concentration of 5 mg/ml or 0. 5 mg/ml could prevent photodamage of the photoageing model in the dermis, and Tempol at the concentration of 5 mg/ml had stronger protective effects. Conclusions Guinea pig can be applied as an useful animal model of skin photoageing. Antioxidant tempol has photoprotective effects on photodamage of the photoageing model in guinea pig and can be used as an anti-photoageing agent.
