CAJ | 학술논문

目的探讨与胰腺内皮细胞共培养时C3A细胞功能变化.方法选择C3A细胞与胰腺内皮细胞的密度比例为10:1分别进行直接和间接共培养7 d,同时设立空白对照组,观察各实验组细胞生长和形态特征,分别用杜邦全自动生化分析仪、放射免疫分析法和ELISA法检测培养液中AST、ALT漏出量,白蛋白含量和安定代谢量.结果 C3A细胞与胰腺内皮细胞直接共培养第5、7天能显著降低ALT、AST的漏出量,第7天提高白蛋白合成量最高达12.977μg/ml和提高安定代谢能力;C3A细胞与胰腺内皮细胞间接共培养在第5、7天也能显著提高白蛋白合成量到7.380μg/ml、10.773μg/ml.结论与胰腺内皮细胞直接与间接共培养均能明显改善C3A细胞的功能.
목적 탐토여이선내피세포공배양시C3A세포공능변화.방법 선택C3A세포여이선내피세포적밀도비례위10:1분별진행직접화간접공배양7 d,동시설립공백대조조,관찰각실험조세포생장화형태특정,분별용두방전자동생화분석의、방사면역분석법화ELISA법검측배양액중AST、ALT루출량,백단백함량화안정대사량.결과 C3A세포여이선내피세포직접공배양제5、7천능현저강저ALT、AST적루출량,제7천제고백단백합성량최고체12.977μg/ml화제고안정대사능력;C3A세포여이선내피세포간접공배양재제5、7천야능현저제고백단백합성량도7.380μg/ml、10.773μg/ml.결론 여이선내피세포직접여간접공배양균능명현개선C3A세포적공능.
Objective To investigate the functional changes of C3A cells co-cultured with pancreatic endothelial cells. Methods C3A cells and pancreatic endothelial cells lwere cultivated directly and indirectly for 7 days by the density ratio of 10: 1. Meanwhile, the blank control was established.The growth and morphological characteristics of experimental groups were observed. The out leakage level of AST, ALT and the synthesis level of albumin and the diazepam metabolism level were determined by automatic biochemistry analyzer, radioimmunoassay and enzyme linked immunosorbent assay. Results Direct co-cultivation of C3A cells and pancreatic endothelial cells could reduce the out leakage of ALT and AST in the 5th and 7th d. The capacity of albumin synthesis could reach 12. 977μg/ml on the 7th d and the that of diazepam metabolism could also be improved. Indirect co-cultivation of C3A cells and pancreatic endothelial cells could significantly raise the albumin synthesis to 7. 380 μg/ml and 10. 773 μg/ml on the 5th and 7th d, respectively. Conclusion Co-cultivation of C3A cells and pancreatic endothelial cells can significantly improve the basic biological function of C3A cells.