CAJ | 학술논문

本文成功地应用粗制pBR322-HBV DNA制备滤膜,进行逆向斑点杂交,从而进一步简化了方法,省却了CsCl超离心纯化重组质粒的繁复过程.为该方法商品化创造了条件.通过双盲法,收集临床肝炎血清进行逆向斑点杂交测定,对具有免疫血清标志及正向HBV DNA斑点杂交资料的383例血清的分析,发现69例:KBsAg(+)/HBeAg(+)/DNA正向斑试(+)血清100%显示阳性结果;28例HBsAg(+)/HBeAg(一)/DNA正向斑试(+)中有23例呈阳性反应;受检的28例各项HBV血清标志皆阴性的非乙型肝炎的肝病患者(其中11例为甲型肝炎,6例为非甲非乙型肝炎)逆向斑点杂交全部阴性,从而证明该方法确为灵敏、可靠的确定HBV感染性的有效手段,在乙型肝炎的诊断与防治中有重要的实用价值.
본문성공지응용조제pBR322-HBV DNA제비려막,진행역향반점잡교,종이진일보간화료방법,성각료CsCl초리심순화중조질립적번복과정.위해방법상품화창조료조건.통과쌍맹법,수집림상간염혈청진행역향반점잡교측정,대구유면역혈청표지급정향HBV DNA반점잡교자료적383례혈청적분석,발현69례:KBsAg(+)/HBeAg(+)/DNA정향반시(+)혈청100%현시양성결과;28례HBsAg(+)/HBeAg(일)/DNA정향반시(+)중유23례정양성반응;수검적28례각항HBV혈청표지개음성적비을형간염적간병환자(기중11례위갑형간염,6례위비갑비을형간염)역향반점잡교전부음성,종이증명해방법학위령민、가고적학정HBV감염성적유효수단,재을형간염적진단여방치중유중요적실용개치.
The assay of HBV DNA reverse spot hybridization has been more simplified by using the filter with crude pBR322-HBV DNA spot,which was prepared by NaOH/SDS method.As a marker of HBV infection.the serum of hepatitis patients from several hospitals in Shanghai were assayed.Three hundred eighty-three samples of hepatitis patients with HBV immunoserological markers and HBV DNA spot assay were analysed by double blind test.It indicated that all the 63 cases of HBsAg(+)/HBoAg(+)/HBV DNA(q-)and 23 out of the 28 cases of HBsAg(q-)/HBoAg(-)/HBV DNA(+)showed positive reaction;28 cases of non-HBV hepatitis including 11 HAV and 6 NANB patients were negative.It is proved that this assay is a sensitive dependable marker for identifying the infectious status of HBV carriers.