CAJ | 학술논문

目的调查广东地区TT病毒(TTV)感染流行情况和基因分型.方法选取TTVORF1的保守序列作内外引物,Ⅰ型、Ⅱ型同源性极高的序列nt2464～nt2520作包被探针,异源性大于50%的序列nt2125～nt2159作显色探针Ⅰ和显色探针Ⅱ,采用微板核酸杂交-ELISA技术对280例肝功能损害的肝炎患者血清进行TTV检测及TTV基因分型研究.结果检测出44例TTV阳性患者,检出率为15.71%.对44例TTV阳性患者的标本进行分型,Ⅰ型、Ⅱ型和混合型比例为30:4:10.慢性肝炎中TTV感染者与急性肝炎中TTV感染者比例为28:16.结论在广东地区的肝炎疾病中,TTV有较高的发病率,且慢性肝炎感染TTV的机会多于急性肝炎,TTV可分为两个型:Ⅰ型和Ⅱ型,Ⅰ型占68.18%,Ⅱ型占9.09%,Ⅰ型明显多于Ⅱ型.微板核酸杂交-ELISA技术特异性强,灵敏度高,分型准确快速,在临床上具有实用价值.
목적 조사엄동지구TT병독(TTV)감염류행정황화기인분형.방법 선취TTVORF1적보수서렬작내외인물,Ⅰ형、Ⅱ형동원성겁고적서렬nt2464～nt2520작포피탐침,이원성대우50%적서렬nt2125～nt2159작현색탐침Ⅰ화현색탐침Ⅱ,채용미판핵산잡교-ELISA기술대280례간공능손해적간염환자혈청진행TTV검측급TTV기인분형연구.결과 검측출44례TTV양성환자,검출솔위15.71%.대44례TTV양성환자적표본진행분형,Ⅰ형、Ⅱ형화혼합형비례위30:4:10.만성간염중TTV감염자여급성간염중TTV감염자비례위28:16.결론 재엄동지구적간염질병중,TTV유교고적발병솔,차만성간염감염TTV적궤회다우급성간염,TTV가분위량개형:Ⅰ형화Ⅱ형,Ⅰ형점68.18%,Ⅱ형점9.09%,Ⅰ형명현다우Ⅱ형.미판핵산잡교-ELISA기술특이성강,령민도고,분형준학쾌속,재림상상구유실용개치.
Objective To study the prevalence and genotypes of TT virus(TTV)infection in Guangdong China,Methods Two pairs of primers derived from open reading frame 1(ORF1)of the TT virus genome,capture probe from nt2464～nt2520,a conserved region of the TTV genome,and detector probe from nt2125～nt2159 were used to detect 280 sera samples of hepatitis patients by Microplate Sandwich Hybridization-ELISA technique.Results Forty foLlr(15,71%)of 280 hepatitis patients were positive for TTV DNA.68.18%of which showed genotype Ⅰ infections,9.09%showed genotype Ⅱ infections and 27.73%were mixed genotype Ⅰ/Ⅱ infections.There were 28 chronic hepatitis patients and 16 acute hepatitis patients in 44 TTV positive cases.Conclusion In GLlangdong China.there was higher prevalence of TT virus infection in hepatitis patients,especially chronic hepatitis patients.There were two genotypes of TTV:68.18%of genotype Ⅰ and 9.09%of genotype Ⅱ.Microplate sandwich hybridization-ELISA technique is a sensitive and specific method in studying the prevalene and genotypes of TT virus.