目的 探讨膳食碘摄入量对适钠和低钠饮食小鼠血脂代谢影响,为揭示碘与心血管疾病发病的相关性进行初步研究.方法 Balb/c小鼠260只,按体质量、性别随机分为适钠组(Na)和低钠组(Lna),每组130只;此两大组又分别按照碘摄入量分为①重度低碘组(SID);②轻度低碘组(MID);③适碘组(NI);④10倍碘过量组(10HI);⑤50倍碘过量组(50HI),总计为10组,每组26只.各组小鼠在饲养8个月时收集尿液和小鼠外周血,分离血清.测定小鼠尿碘、体质量、血脂[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白醇(HDL)]及甲状腺激素水平[总甲状腺素(TT4),总三碘甲腺原氨酸(TT3)、游离甲状腺素(FT4)、游离三碘甲腺原氨酸(FT3)].结果适钠组中,SID组、MID组雄性小鼠TG水平[(1.64±0.35)、(1.67±0.31)mmol/L]和SID组TC水平[(3.88±0.35)mmol/L]明显高于NI组[(1.49±0.42)、(3.25±0.47)mmol/L,P均＜0.05],SID组中雌性小鼠TG水平[(1.52±0.22)mmol/L]高于NI组[(1.23±0.22)mmol/L,P＜0.05].10HI和50HI组雄性小鼠外周血TG水平[(1.16±0.23)、(1.21±0.27)mmol/L]低于NI组(P均＜0.05),雌性小鼠TC水平[(2.37±0.49)、(2.48±0.37)mmol/L)低于NI组[(2.84±0.37)mmol/L,P均＜0.05].在低钠组,SID组雄性小鼠TG、TC水平[(1.39±0.40)、(3.33±0.46)mmol/L]均高于NI组[(1.30±0.28)、(3.00±0.53)mmol/L,P均＜0.05],SID组雌性小鼠TG、TC、LDL水平[(1.48±0.26)、(276±0.43)、(0.62±0.22)mmol/L]、MID组雌性小鼠LDL水平[(0.60±0.17)mmol/L]均高于NI组[(1.22±0.36)、(2.51±0.38)、(0.48±0.08)mmol/L,P均＜0.05].10HI和50HI组雄性小鼠TG水平[(1.12±0.22)、(0.90±0.11)mmol/L]均低于NI组(P均＜0.05),10HI和50HI组雌性小鼠TC水平[(2.35±0.34)、(2.37±0.37)mmol/L]、50HI组雌性小鼠LDL水平[(0.65±0.18)mmol/L]均低于NI组(P均＜0.05).适钠组中的SID和MID组血清TT4[(0.00±0.00)、(17.15±15.26)nmol/L]、FT4[(0.93±0.42)、(18.46±4.31)pmol/L]和TT3[(0.49±0.07)、(0.67±0.10)nmol/L]、FT3[(2.86±0.37)、(3.18±0.24)pmol/L]水平均低于NI组[(37.15±15.26)、(28.46±4.31)、(0.85±0.10)、(3.87±0.24)pmol/L,P＜0.01或P＜0.05].在低钠组,SID和MID组血清TT4、FT4和TT3、FT3水平[(0.00±0.00)nmol/L、(1.03±0.78)pmol/L、(0.51±0.05)nmol/L,(3.01±0.17)pmol/L,(19.76±12.22)nmol/L、(21.46±5.37)pmol/L、(0.71±0.21)nmol/L、(3.56±0.23)pmol/L]均低于NI组[(36.23±14.72)nmol/L、(30.96±6.33)pmol/L、(0.89±0.20)nmol/L、(4.05±0.24)pmol/L,P均＜0.05],10HI组的TT3水平[(1.06±0.23)nmol/L]高于NI组(P＜0.05).结论碘缺乏可致TG、TC和LDL升高,过量碘可致TG/TC水平降低.碘摄入量是影响血脂代谢的重要因素.限盐饮食的同时,严密监控碘的摄入量对当前有效防治心血管疾病应具有重要作用.
目的 探討膳食碘攝入量對適鈉和低鈉飲食小鼠血脂代謝影響,為揭示碘與心血管疾病髮病的相關性進行初步研究.方法 Balb/c小鼠260隻,按體質量、性彆隨機分為適鈉組(Na)和低鈉組(Lna),每組130隻;此兩大組又分彆按照碘攝入量分為①重度低碘組(SID);②輕度低碘組(MID);③適碘組(NI);④10倍碘過量組(10HI);⑤50倍碘過量組(50HI),總計為10組,每組26隻.各組小鼠在飼養8箇月時收集尿液和小鼠外週血,分離血清.測定小鼠尿碘、體質量、血脂[甘油三酯(TG)、總膽固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白醇(HDL)]及甲狀腺激素水平[總甲狀腺素(TT4),總三碘甲腺原氨痠(TT3)、遊離甲狀腺素(FT4)、遊離三碘甲腺原氨痠(FT3)].結果適鈉組中,SID組、MID組雄性小鼠TG水平[(1.64±0.35)、(1.67±0.31)mmol/L]和SID組TC水平[(3.88±0.35)mmol/L]明顯高于NI組[(1.49±0.42)、(3.25±0.47)mmol/L,P均＜0.05],SID組中雌性小鼠TG水平[(1.52±0.22)mmol/L]高于NI組[(1.23±0.22)mmol/L,P＜0.05].10HI和50HI組雄性小鼠外週血TG水平[(1.16±0.23)、(1.21±0.27)mmol/L]低于NI組(P均＜0.05),雌性小鼠TC水平[(2.37±0.49)、(2.48±0.37)mmol/L)低于NI組[(2.84±0.37)mmol/L,P均＜0.05].在低鈉組,SID組雄性小鼠TG、TC水平[(1.39±0.40)、(3.33±0.46)mmol/L]均高于NI組[(1.30±0.28)、(3.00±0.53)mmol/L,P均＜0.05],SID組雌性小鼠TG、TC、LDL水平[(1.48±0.26)、(276±0.43)、(0.62±0.22)mmol/L]、MID組雌性小鼠LDL水平[(0.60±0.17)mmol/L]均高于NI組[(1.22±0.36)、(2.51±0.38)、(0.48±0.08)mmol/L,P均＜0.05].10HI和50HI組雄性小鼠TG水平[(1.12±0.22)、(0.90±0.11)mmol/L]均低于NI組(P均＜0.05),10HI和50HI組雌性小鼠TC水平[(2.35±0.34)、(2.37±0.37)mmol/L]、50HI組雌性小鼠LDL水平[(0.65±0.18)mmol/L]均低于NI組(P均＜0.05).適鈉組中的SID和MID組血清TT4[(0.00±0.00)、(17.15±15.26)nmol/L]、FT4[(0.93±0.42)、(18.46±4.31)pmol/L]和TT3[(0.49±0.07)、(0.67±0.10)nmol/L]、FT3[(2.86±0.37)、(3.18±0.24)pmol/L]水平均低于NI組[(37.15±15.26)、(28.46±4.31)、(0.85±0.10)、(3.87±0.24)pmol/L,P＜0.01或P＜0.05].在低鈉組,SID和MID組血清TT4、FT4和TT3、FT3水平[(0.00±0.00)nmol/L、(1.03±0.78)pmol/L、(0.51±0.05)nmol/L,(3.01±0.17)pmol/L,(19.76±12.22)nmol/L、(21.46±5.37)pmol/L、(0.71±0.21)nmol/L、(3.56±0.23)pmol/L]均低于NI組[(36.23±14.72)nmol/L、(30.96±6.33)pmol/L、(0.89±0.20)nmol/L、(4.05±0.24)pmol/L,P均＜0.05],10HI組的TT3水平[(1.06±0.23)nmol/L]高于NI組(P＜0.05).結論碘缺乏可緻TG、TC和LDL升高,過量碘可緻TG/TC水平降低.碘攝入量是影響血脂代謝的重要因素.限鹽飲食的同時,嚴密鑑控碘的攝入量對噹前有效防治心血管疾病應具有重要作用.
목적 탐토선식전섭입량대괄납화저납음식소서혈지대사영향,위게시전여심혈관질병발병적상관성진행초보연구.방법 Balb/c소서260지,안체질량、성별수궤분위괄납조(Na)화저납조(Lna),매조130지;차량대조우분별안조전섭입량분위①중도저전조(SID);②경도저전조(MID);③괄전조(NI);④10배전과량조(10HI);⑤50배전과량조(50HI),총계위10조,매조26지.각조소서재사양8개월시수집뇨액화소서외주혈,분리혈청.측정소서뇨전、체질량、혈지[감유삼지(TG)、총담고순(TC)、저밀도지단백(LDL)、고밀도지단백순(HDL)]급갑상선격소수평[총갑상선소(TT4),총삼전갑선원안산(TT3)、유리갑상선소(FT4)、유리삼전갑선원안산(FT3)].결과괄납조중,SID조、MID조웅성소서TG수평[(1.64±0.35)、(1.67±0.31)mmol/L]화SID조TC수평[(3.88±0.35)mmol/L]명현고우NI조[(1.49±0.42)、(3.25±0.47)mmol/L,P균＜0.05],SID조중자성소서TG수평[(1.52±0.22)mmol/L]고우NI조[(1.23±0.22)mmol/L,P＜0.05].10HI화50HI조웅성소서외주혈TG수평[(1.16±0.23)、(1.21±0.27)mmol/L]저우NI조(P균＜0.05),자성소서TC수평[(2.37±0.49)、(2.48±0.37)mmol/L)저우NI조[(2.84±0.37)mmol/L,P균＜0.05].재저납조,SID조웅성소서TG、TC수평[(1.39±0.40)、(3.33±0.46)mmol/L]균고우NI조[(1.30±0.28)、(3.00±0.53)mmol/L,P균＜0.05],SID조자성소서TG、TC、LDL수평[(1.48±0.26)、(276±0.43)、(0.62±0.22)mmol/L]、MID조자성소서LDL수평[(0.60±0.17)mmol/L]균고우NI조[(1.22±0.36)、(2.51±0.38)、(0.48±0.08)mmol/L,P균＜0.05].10HI화50HI조웅성소서TG수평[(1.12±0.22)、(0.90±0.11)mmol/L]균저우NI조(P균＜0.05),10HI화50HI조자성소서TC수평[(2.35±0.34)、(2.37±0.37)mmol/L]、50HI조자성소서LDL수평[(0.65±0.18)mmol/L]균저우NI조(P균＜0.05).괄납조중적SID화MID조혈청TT4[(0.00±0.00)、(17.15±15.26)nmol/L]、FT4[(0.93±0.42)、(18.46±4.31)pmol/L]화TT3[(0.49±0.07)、(0.67±0.10)nmol/L]、FT3[(2.86±0.37)、(3.18±0.24)pmol/L]수평균저우NI조[(37.15±15.26)、(28.46±4.31)、(0.85±0.10)、(3.87±0.24)pmol/L,P＜0.01혹P＜0.05].재저납조,SID화MID조혈청TT4、FT4화TT3、FT3수평[(0.00±0.00)nmol/L、(1.03±0.78)pmol/L、(0.51±0.05)nmol/L,(3.01±0.17)pmol/L,(19.76±12.22)nmol/L、(21.46±5.37)pmol/L、(0.71±0.21)nmol/L、(3.56±0.23)pmol/L]균저우NI조[(36.23±14.72)nmol/L、(30.96±6.33)pmol/L、(0.89±0.20)nmol/L、(4.05±0.24)pmol/L,P균＜0.05],10HI조적TT3수평[(1.06±0.23)nmol/L]고우NI조(P＜0.05).결론전결핍가치TG、TC화LDL승고,과량전가치TG/TC수평강저.전섭입량시영향혈지대사적중요인소.한염음식적동시,엄밀감공전적섭입량대당전유효방치심혈관질병응구유중요작용.
Objective The present study has been designed to investigate the impact of dietary iodine/sodium intake on blood lipid metabolism in mice. Methods According to body weight and gender, two hundred and sixty Balb/c mice were randomly divided into 2 groups including normal sodium group(Na) and low sodium group(LNa), with 130 animals per group. Each group were then randomly further divided into 5 sub-groups according to the amount of iodine intake: ① severe iodine deficiency(SID); ② mild iodine deficiency(MID); (③normal iodine (NI); ④ 10-fold high iodine ( 10HI ); (⑤ 50-fold high iodine (50HI), 10 groups in total, 26 per group.Eight months later, the body weight and the levels of urinary iodine, thyroid hormones and total cholesterol (TC),Results In Na group, the levels of TG and TC in male mice of SID group[ (1.64 ± 0.35), (3.88 ± 0.35 )mmol/L]and MID group[ ( 1.67 ± 0.31 ), (3.41 ± 0.66)mmol/L] were significantly higher than that of NI group[ ( 1.49 ± 0.42), (3.25 ± 0.47)mmol/L] and the levels of TG in female mice of SID group[(1.52 ± 0.22)mmol/L] were significantly higher than that of NI group[ (1.23 ± 0.22)mmol/L]. In addition, the levels of TG in male mice of 10HI and 50HI groups [ ( 1.16 ± 0.23 ), ( 1.21 ± 0.27 ) mmol/L ] were significantly lower than that of NI group [ ( 1.49 ± 0.42)mmol/L, all P ＜ 0.05], the levels of TC in female mice of 10HI and 50HI groups[(2.37 ± 0.49), (2.48 ± 0.37)mmol/L] were significantly lower than that of NI group[ (2.84 ± 0.37) mmol/L, all P ＜ 0.05 ]. In LNa group,the levels of TG and TC in male mice of SID group[ (1.39 ± 0.40), (3.33 ± 0.46 )mmol/L] were significantly lower than that of NI group [(1.30 ± 0.28), (3.00 ± 0.53) mmol/L, all P ＜ 0.05], the levels of TG, TC and LDL in female mice of SID group[ (1.48 ± 0.26), (2.76 ± 0.43), (0.62 ± 0.22)mmol/L], the levels of LDL in female mice of MID group[ (0.60 ± 0.17 )mmol/L] were significantly lower than that of NI group[(l.22 ± 0.36), (2.51 ± 0.38),(0.48 ± 0.08), (0.48 ± 0.08)mmol/L, all P ＜ 0.05], the levels of TG in male mice of 10HI and 50HI group [ (1.12 ± 0.22), (0.90 ± 0.11 )mmol/L] were significantly lower than that of NI group (all P ＜ 0.05 ), the levels of TC in female mice of 10HI and 50HI groups[ (2.35 ± 0.34), (2.37 ± 0.37)mmol/L], the levels of LDL in female mice of 50HI group[(0.65 ± 0.18)mmol/L], were significantly lower than that of NI group(all P ＜ 0.05). In Na group, the levels of thyroid hormones were distinctively decreased in SID group[TT4(0.00 ± 0.00)nmol/L, FT4 (0.93 ± 0.42)pmol/L, TT3(0.49 ± 0.07)nmol/L, FT3(2.86 ± 0.37)pmol/L] and MID group [TT4 (17.15 ± 15.26)nmol/L, FT4( 18.46 ± 4.31 )pmol/L, TT3(0.67 ± 0. 10)nmol/L, FT3(3.18 ± 0.24)pmol/L] compared with that of the NI group [TT4 (37.15 ± 15.26)nmol/L, FT4(28.46 ± 4.31)pmol/L, TT3(0.85 ± 0.10)pmol/L, FT3(3.87 ± 0.24)pmol/L, all P ＜ 0.05 ]. In LNa group, the levels of thyroid hormones were distinctively decreased in SID group [TT4 (0.00 ± 0.00) nmol/L,FT4(1.03 ± 0.78)pmol/L, TT3(0.51 ± 0.05)nmol/L, FT3(3.01 ± 0.17)pmol/L] and MID group[TT4(19.76 ± 12.22)nmol/L, FT4(21.46 ± 5.37)pmol/L, TT3(0.71 ± 0.21)nmol/L, FT3(3.56 ± 0.23)pmol/L] compared with that of the NI group[TT4(36.23 ± 14.72)nmol/L, FT4(30.96 ± 6.33)pmol/L, TT3(0.89 ± 0.20)nmol/L, FT3(4.05 ± 0.24)pmol/L, all P ＜ 0.05]. Conclusions Dietary iodine intake plays an important role in the blood lipid metabolism. Iodine deficiency could increase while iodine excess could decrease the levels of serum TG, TC or LDL in mice. Monitoring the amount of iodine intake during sodium restriction should have an important role in effective prevention and treatment of cardiovascular disease.
