CAJ | 학술논문

目的探讨外源性髓鞘碱性蛋白（myelin basic protein, MBP）在家兔面神经再生室修复中的作用。方法将33只家兔横断的面神经干近、远端缝于硅胶管壁上，形成约12 μL大小的神经再生室。一侧为实验组，将MBP注入再生室内；对侧为对照组不注任何物质。分别在术后4、6、8周处死动物, 切取标本，在光镜下行组织形态学观察。结果形态学分析表明术后4周2组再生室内再生面神经的有髓轴突直径、髓鞘厚度及长入再生室远端有髓轴突数差异无显著性 (P>0.05)，随着时间的延长(术后6、8周)，MBP组较对照组再生面神经显得更为成熟，6周时再生轴突成熟程度差异更明显。结论 MBP有促进家兔面神经再生修复的作用，但在活体内增强受损轴突生长的机理以及MBP促进FN轴突再生的作用时间尚不清楚。
목적탐토외원성수초감성단백（myelin basic protein, MBP）재가토면신경재생실수복중적작용。방법장33지가토횡단적면신경간근、원단봉우규효관벽상，형성약12 μL대소적신경재생실。일측위실험조，장MBP주입재생실내；대측위대조조불주임하물질。분별재술후4、6、8주처사동물, 절취표본，재광경하행조직형태학관찰。결과형태학분석표명술후4주2조재생실내재생면신경적유수축돌직경、수초후도급장입재생실원단유수축돌수차이무현저성 (P>0.05)，수착시간적연장(술후6、8주)，MBP조교대조조재생면신경현득경위성숙，6주시재생축돌성숙정도차이경명현。결론 MBP유촉진가토면신경재생수복적작용，단재활체내증강수손축돌생장적궤리이급MBP촉진FN축돌재생적작용시간상불청초。
Objective　To study the role of exogenous myelin basic protein(MBP) in neural repairment. Methods　Adult New Zealand rabbits were employed in vivo preparation. A 12 μL nerve growth chamber was created by suturing the proximal and distal stumps of a transected facial never (FN) trunk into a tube. The regenerated nerves within the chambers were dissected and fixed for histological studies with light microscope at 4,6 and 8 weeks respectively following the surgery. Results　Morphological analysis of nerves showed no difference between the MBP and control group in the size of the regeneration FN within the chambers, diameters of myelinated axons, thickness of myelin sheath and number of myelin axons grew into the distal end of chamber at 4 weeks. At 6 and 8 weeks after operation, the MBP group showed a more mature-appearance regenerative nerve comparing to control group. Especially, the enhancement of maturation in the regeneration axons was very noticeable at 6 weeks. Conclusion　The study showed that pharmacological administration of exogenous MBP within a chamber at the time of entubational nerve repair enhances regeneration of myelinated axons across the sectioned ends of FN.