CAJ | 학술논문

目的观察普鲁卡因和利多卡因对细胞内织网内ryanodine 敏感的钙离子释放的影响.方法采用60-80 g 的蒙古鼠海马切片,用显微荧光法测定细胞内钙离子浓度.将切片用含50 mmo l/L KCl的人工脑脊液灌注30秒,然后用人工脑脊液灌注,5分钟后用含20 mmol/L 咖啡因的脑脊液灌注2分钟, 然后换为人工脑脊液直到实验结束.将100 umol/L 的普鲁卡因或利多卡因分别加入到高钾后的灌注液中观察它们对钙离子释放的影响.结果咖啡因可致细胞内钙离子浓度的明显增高, 而普鲁卡因可使这种增高减少12%,而利多卡因则无此抑制作用.结论普鲁卡因可抑制ryanodine 受体介导的细胞内钙离子释放,而利多卡因则可能通过其他机理抑制细胞内钙离子的释放.
목적관찰보로잡인화리다잡인대세포내직망내ryanodine 민감적개리자석방적영향.방법채용60-80 g 적몽고서해마절편,용현미형광법측정세포내개리자농도.장절편용함50 mmo l/L KCl적인공뇌척액관주30초,연후용인공뇌척액관주,5분종후용함20 mmol/L 가배인적뇌척액관주2분종, 연후환위인공뇌척액직도실험결속.장100 umol/L 적보로잡인혹리다잡인분별가입도고갑후적관주액중관찰타문대개리자석방적영향.결과가배인가치세포내개리자농도적명현증고, 이보로잡인가사저충증고감소12%,이리다잡인칙무차억제작용.결론보로잡인가억제ryanodine 수체개도적세포내개리자석방,이리다잡인칙가능통과기타궤리억제세포내개리자적석방.
Objective To examine the effects of procaine and lidocaine on intracellular Ca2+ release from sarcoplasmic reticulum ryanodine-sensitive Ca2+ stores. Methods The experiment was performed on hippocampal slices from 60-80g male Mongoliang erbils. Levels of intracellular Ca2+ concentration in the slices were me asured by microfluorometry. The slices were perfused with 50 mmol/L KCl contai ning medium for 30 seconds. Then, the medium was switched to physiological med ium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was s uperfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 μmol/L) on caffeine-evoked Ca2+ release were evaluated by adding them to the medium after high K+ medium perfusion. Results Caffeine induced a marked increase in intracellular Ca2+ concentration which was then decreased 12% upon the addition of procaine (P<0.05); however, lidocaine, did not induce a similar inhibitory reaction. Conclusion Procaine inhibits ryanodine-receptor mediated Ca2+ release from intracell ular Ca2+ stores, while lidocaine may inhibit Ca2+ release through o ther mechanisms.