广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2011年
20期
92-94
,共3页
黄曲霉毒素G2、G1、B2、B1%高效液相色谱-串联质谱%水产品
黃麯黴毒素G2、G1、B2、B1%高效液相色譜-串聯質譜%水產品
황곡매독소G2、G1、B2、B1%고효액상색보-천련질보%수산품
aflatoxin G2%G1%B2%B1%high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）%aquatic products
建立了用高效液相色谱-串联质谱法（HPLC-MS/MS）测定水产品中黄曲霉毒素G2、G1、B2、B1残留量的方法。84%甲醇水溶液提取水产品中4种黄曲霉毒素,正己烷脱脂,HLB固相萃取柱净化。采用电喷雾电离,正离子扫描,选择多反应检测模式（MRM）监测,外标法定量。该法对4种黄曲霉毒素标准曲线的线性回归系数均在0.99以上,黄曲霉毒素G1、B1方法定量限为0.5μg/kg,G2、B2方法定量限为0.3μg/kg。4种黄曲霉毒素的回收率为56%～80%,相对标准偏差1.58%～14.4%。该法灵敏,结果可靠,可用于水产品中黄曲霉毒素的测定。
建立瞭用高效液相色譜-串聯質譜法（HPLC-MS/MS）測定水產品中黃麯黴毒素G2、G1、B2、B1殘留量的方法。84%甲醇水溶液提取水產品中4種黃麯黴毒素,正己烷脫脂,HLB固相萃取柱淨化。採用電噴霧電離,正離子掃描,選擇多反應檢測模式（MRM）鑑測,外標法定量。該法對4種黃麯黴毒素標準麯線的線性迴歸繫數均在0.99以上,黃麯黴毒素G1、B1方法定量限為0.5μg/kg,G2、B2方法定量限為0.3μg/kg。4種黃麯黴毒素的迴收率為56%～80%,相對標準偏差1.58%～14.4%。該法靈敏,結果可靠,可用于水產品中黃麯黴毒素的測定。
건립료용고효액상색보-천련질보법（HPLC-MS/MS）측정수산품중황곡매독소G2、G1、B2、B1잔류량적방법。84%갑순수용액제취수산품중4충황곡매독소,정기완탈지,HLB고상췌취주정화。채용전분무전리,정리자소묘,선택다반응검측모식（MRM）감측,외표법정량。해법대4충황곡매독소표준곡선적선성회귀계수균재0.99이상,황곡매독소G1、B1방법정량한위0.5μg/kg,G2、B2방법정량한위0.3μg/kg。4충황곡매독소적회수솔위56%～80%,상대표준편차1.58%～14.4%。해법령민,결과가고,가용우수산품중황곡매독소적측정。
The method for determining aflatoxin G2,G1,B2,and B1 by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was developed.The samples were extracted by 84% methanol,degreased with hexane and purified with HLB solid-phase extraction column.In addition,with electrospray ionization in positive mode,the samples was monitored with multiple reaction monitoring（MRM） and quantified them with external standard method.For the 4 aflatoxins,it showed good linear regression coeffcient in the standard curve（all0.99）,the limits of detection for aflatoxin G1,B1 were 0.5 μg/kg,and the limits of detection for aflatoxin G2,B2 were 0.3 μg/kg.The average recovery was 56%~80%,and the relative standard deviation was 1.58%~14.4%.The method,was sensitive and reliable,and can be applied for the determination of aflatoxins in aquatic products.
