中国食品工业
中國食品工業
중국식품공업
CHINA FOOD INDUSTRY
2011年
8期
64-67
,共4页
谷胱甘肽%稳定性%分离%超滤技术%功能性食品添加剂
穀胱甘肽%穩定性%分離%超濾技術%功能性食品添加劑
곡광감태%은정성%분리%초려기술%공능성식품첨가제
glutathione%separation%ultra-filtration%functional food additive
本文对从酵母细胞中抽提的谷胱甘肽(GSH)的稳定性和超滤技术(UF)对GSH抽提液的分离纯化进行了研究。研究结果表明,在实际体系中影响GSH稳定性的主要因素是pH值和温度:pH值为2.0-4.0范围内最为稳定,GSH在30℃下,24h自身氧化较少;UF去除GSH抽提液中的蛋白质有很好效果,最佳工艺参数为:△P=0.15MPa、T=25℃、pH=2-3,通过间歇稀释过滤回收率达到98%以上。
本文對從酵母細胞中抽提的穀胱甘肽(GSH)的穩定性和超濾技術(UF)對GSH抽提液的分離純化進行瞭研究。研究結果錶明,在實際體繫中影響GSH穩定性的主要因素是pH值和溫度:pH值為2.0-4.0範圍內最為穩定,GSH在30℃下,24h自身氧化較少;UF去除GSH抽提液中的蛋白質有很好效果,最佳工藝參數為:△P=0.15MPa、T=25℃、pH=2-3,通過間歇稀釋過濾迴收率達到98%以上。
본문대종효모세포중추제적곡광감태(GSH)적은정성화초려기술(UF)대GSH추제액적분리순화진행료연구。연구결과표명,재실제체계중영향GSH은정성적주요인소시pH치화온도:pH치위2.0-4.0범위내최위은정,GSH재30℃하,24h자신양화교소;UF거제GSH추제액중적단백질유흔호효과,최가공예삼수위:△P=0.15MPa、T=25℃、pH=2-3,통과간헐희석과려회수솔체도98%이상。
The stability of reduced glutathione (GSH) in extracting solution from Candida utilis cell was studied by pH and temperature. And ultra-filtration (UF) for removing protein from GSH extraction was determined in this paper. Results indicated that GSH extracting solution was relative stabile under the following conditions, i.e., pH = 2.0-4.0, temperature 〈 30 ℃. And UF was an optimal selection for removing protein from GSH under △P=0.15MPa, T=20℃, pH = 2.0-3.0. Others the recovery rate GSH was over 98% by discontinuous dilution during the late stage of UF.