CAJ | 학술논문

为鉴定维氏气单胞菌（Aeromonas veronii,AV）吉林分离株合适的诊断或免疫用抗原,研究依据GenBank维氏气单胞菌外膜蛋白AⅠ基因（OMPAⅠ）设计引物,通过PCR成功扩增出长度为1 026 bp的基因片段,通过序列分析表明：该基因与维氏气单胞菌AB2902300 OMPAⅠ的同源性为93%,与其他气单胞菌同源性大于80%;同时通过生物信息学软件对其蛋白的分子特征进行了预测,为OMPAⅠ可能做为诊断或免疫用抗原提供了理论依据。
위감정유씨기단포균（Aeromonas veronii,AV）길림분리주합괄적진단혹면역용항원,연구의거GenBank유씨기단포균외막단백AⅠ기인（OMPAⅠ）설계인물,통과PCR성공확증출장도위1 026 bp적기인편단,통과서렬분석표명：해기인여유씨기단포균AB2902300 OMPAⅠ적동원성위93%,여기타기단포균동원성대우80%;동시통과생물신식학연건대기단백적분자특정진행료예측,위OMPAⅠ가능주위진단혹면역용항원제공료이론의거。
To determine the proper diagnostic or immune antigen of Aeromonas veronii isolated from Jilin,the OMPA I for 1 026 bp was amplified by PCR using a couple of primers designed according to the gene sequence reported by Gene Bank.The sequence analysis showed its homology with the OMPA I of Aeromonas veronii AB2902300 reached 93% and others exceeded 80%.Meanwile,the molecular characteristics of protein was predicted by bioinformatics software and offered the theoretical basis for the OMPA I as possible diagnostic or immune antigen.