CAJ | 학술논문

为建立一种快速、高效的诊断家畜细粒棘球蚴病方法，提取绵羊细粒棘球蚴包囊新鲜囊液，盐析囊液抗原，点样于硝酸纤维膜，以胶体金-驴抗羊IgG和胶体金-兔抗鼠IgG为检测标记物，采用垂直流渗滤装置检测绵羊与人工感染细粒棘球蚴小鼠血清和全血特异性抗体。患病绵羊阳性血清及全血检出率在90．91％～94．4％，细粒棘球蚴感染小鼠血清及全血检出率均为100％；细粒棘球蚴阴性羊血清和全血假阳性率为4．00％-4．59％；与脑多头蚴病血清交叉反应率为28．57％（2／7）。研究结果表明细粒棘球蚴全血金标渗滤法（DIGFA）可应用于绵羊棘球蚴病的诊断与检疫。
위건립일충쾌속、고효적진단가축세립극구유병방법，제취면양세립극구유포낭신선낭액，염석낭액항원，점양우초산섬유막，이효체금-려항양IgG화효체금-토항서IgG위검측표기물，채용수직류삼려장치검측면양여인공감염세립극구유소서혈청화전혈특이성항체。환병면양양성혈청급전혈검출솔재90．91％～94．4％，세립극구유감염소서혈청급전혈검출솔균위100％；세립극구유음성양혈청화전혈가양성솔위4．00％-4．59％；여뇌다두유병혈청교차반응솔위28．57％（2／7）。연구결과표명세립극구유전혈금표삼려법（DIGFA）가응용우면양극구유병적진단여검역。
In order to establish a rapid and high-performance immunoassay assay in whole blood of livestock for diagnosis of hydatidosis, the cyst fluid antigen was extracted from fresh sheep Echinococcus cysts and salted-out by ammonium sulphate.The soluble cyst fluid used as antigen was dotted on nitro-cellulose and colliodal gold-donkey anti-goat IgG and colliodal gold-rabbit anti-mice IgG were employed as the probe, and a filtration device with vertical flow was applied to detect the specific IgG antibodies of sheep and mice infected with protoscoleces of Echinococcus granulosus. By using the new immunoassay, it was found that 90.91%-94.4% of whole blood and serum from sheep and 100% of whole blood and serum from mice were positive for hydatidosis, and 5% of health individuals had false positive results.However, partially high percentages of cross reactions could be demonstrated in serum of sheep with Taenia multiceps.The results showed that the dot immune gold filtration assay （DIGFA） could be used for diagnosis and quarantine inspection of livestock hydatidosis.