CAJ | 학술논문

[目的]探讨中国鹿亚科动物的遗传分化。[方法]通过PCR直接测序的方法获得我国鹿亚科物种线粒体DNA控制区（D-loop）全序列,结合GenBank检索到的鹿亚科其它动物同源序列,用生物软件进行统计分析。[结果]我国鹿亚科动物D-loop区序列全长在921～1072bp,碱基T、A、C、G的平均含量分别为32.1%、30.2%、22.7%及15.0%,各物种间的遗传距离范围在0.062～0.106,处于属间差异。系统进化树结果表明梅花鹿、马鹿和白唇鹿亲缘关系较近,它们与水鹿构成一组进化枝,坡鹿与麋鹿构成一组进化枝,豚鹿单独构成一个进化枝。[结论]支持麋鹿和豚鹿并入鹿属的观点,我国的鹿亚科动物的分化时间约为155～260万年。
[목적]탐토중국록아과동물적유전분화。[방법]통과PCR직접측서적방법획득아국록아과물충선립체DNA공제구（D-loop）전서렬,결합GenBank검색도적록아과기타동물동원서렬,용생물연건진행통계분석。[결과]아국록아과동물D-loop구서렬전장재921～1072bp,감기T、A、C、G적평균함량분별위32.1%、30.2%、22.7%급15.0%,각물충간적유전거리범위재0.062～0.106,처우속간차이。계통진화수결과표명매화록、마록화백진록친연관계교근,타문여수록구성일조진화지,파록여미록구성일조진화지,돈록단독구성일개진화지。[결론]지지미록화돈록병입록속적관점,아국적록아과동물적분화시간약위155～260만년。
[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequences of others Cervinae were gained from GenBank, and then analyzed through biology software. [Result] The lengths of their control region were 921-1 072 bp, the nucleotide content of T, A, C, and G was 32.1%, 30.2%, 22.7% and 15.0% separately. Genetic distance of each species ranged from 0.062-0.106, which belonged to level of inter-genus. The molecular phylogenetic tree indicated that red deer, skia deer and White-lipped Deer had close relationship, and they constituted one branch with sambar deer. Eld＇s deer and elk deer constituted the second branch, and hog deer constituted the third branch alone. [Conclusion] Hog deer and fallow deer should be incorporated in Cervus, divergence time is about 1.55-2.60 million years among Cervinae in China.