CAJ | 학술논문

目的测定不同采收期小叶丁香皮中黄酮类及多糖的含量,对小叶丁香皮的质量进行综合评价。方法采用比色法测定黄酮及多糖含量,以芦丁和葡萄糖为对照品,在500nm波长下测定吸光度,测定黄酮的含量,在620nm波长下测定总多糖吸光度,分别测定可溶性多糖和粗多糖的含量。结果 0.05mg-0.5mg范围内,芦丁质量与吸光度A值线性关系良好,R2=0.9990;在0.052mg～0.20mg范围内,葡萄糖质量与吸光度A值线性关系良好R2=0.9974。黄酮在4月29日含量最高为14.86%,总多糖在6月23日含量最高为33.24%。结论通过T-TEXT比较不同采收期小叶丁香皮中的总黄酮和多糖含量,差异显著,多糖随着黄酮的增加呈上升的趋势。本方法简便、快速、可靠、适合小叶丁香黄酮及多糖的含量测定。
목적측정불동채수기소협정향피중황동류급다당적함량,대소협정향피적질량진행종합평개。방법채용비색법측정황동급다당함량,이호정화포도당위대조품,재500nm파장하측정흡광도,측정황동적함량,재620nm파장하측정총다당흡광도,분별측정가용성다당화조다당적함량。결과 0.05mg-0.5mg범위내,호정질량여흡광도A치선성관계량호,R2=0.9990;재0.052mg～0.20mg범위내,포도당질량여흡광도A치선성관계량호R2=0.9974。황동재4월29일함량최고위14.86%,총다당재6월23일함량최고위33.24%。결론통과T-TEXT비교불동채수기소협정향피중적총황동화다당함량,차이현저,다당수착황동적증가정상승적추세。본방법간편、쾌속、가고、괄합소협정향황동급다당적함량측정。
Purpose： To determine the contents of flavonoids and polysaccharides form skin of Syringa Pubescens Turcz.in different harvest,and evaluates quality control the skin of Syringa Pubescens Turcz..Method： Determination of flavonoids and polysaccharides by chromatometry.The reference standards are rutin and glucose,and determined contents of flavonoids at 500nm wavelength,and determined of total polysaccharides which content soluble polysaccharide and crude polysaccharide at 620nm wavelength.Result： Rutin quality and value of the absorbance A has a good linear relationship within 0.05mg-0.5mg,R2=0.9990;in the range of 0.052mg ~ 0.20mg of glucose and the absorbance value A has a good linear relationship,R2=0.9974.Flavonoid content of the highest in the April 29 up to 14.86%,the total polysaccharide content in the June 23 up to 33.24% Conclusion： The differences of the polysacharides and total flavonoid in the skin of Syringa Pubescens Turcz is Significant difference at different growth stages when texted by T-TEXT.Polysaccharide increased with the flavonoids were rising.This method is simple,fast,reliable and suitable for determination of flavonoids and polysaccharides.