CAJ | 학술논문

针对生物细胞原位检测和提高分类精度这一研究热点,以白细胞为主要研究对象,利用细胞光散射识别方法,根据细胞各方向散射光的特点,设计了一种多维光学传感系统,即是通过该光学系统可以检测细胞前向大小角,侧向偏振动,以及后向散射光。其中对各向光路系统构成和各个元件参数进行了优化设计,使系统的分辨率在80lp/mm时,MTF值不低于0.18,满足检测要求。同时后向散射光的接收光功率提高为原来的2倍,有效滤除杂散光,保证高信噪比信号的提取。优化设计后的多维光学传感系统可应用于白细胞的精确识别科学技术领域。
침대생물세포원위검측화제고분류정도저일연구열점,이백세포위주요연구대상,이용세포광산사식별방법,근거세포각방향산사광적특점,설계료일충다유광학전감계통,즉시통과해광학계통가이검측세포전향대소각,측향편진동,이급후향산사광。기중대각향광로계통구성화각개원건삼수진행료우화설계,사계통적분변솔재80lp/mm시,MTF치불저우0.18,만족검측요구。동시후향산사광적접수광공솔제고위원래적2배,유효려제잡산광,보증고신조비신호적제취。우화설계후적다유광학전감계통가응용우백세포적정학식별과학기술영역。
In order to improve classification precision and in situ detection of biological cells,Leukocyte subclasses are analyzed by light scattering identification method.A multidimensional optical sensing system is designed based on the characteristics of their scattering light.It can detect forward small and large angle scatter,polarized side scatter and back scatter.After optimal design of optic system structure and each element parameters,when the resolution is 80lp/mm,the MTF value is not less than 0.18.The optical efficiency of back receiver system increases 1 time.The stray light is filtered out effectively and scattering signals with a good signal-to-noise ratio can be extracted.The optical system optimized can be used in scientific research of Leukocyte identification.