CAJ | 학술논문

本研究主要探讨不同浓度NaCl胁迫处理下豇豆（Vigna unguiculata Linn.）幼苗叶片抗氧化酶活性的变化情况。研究结果表明,在0～250mmol/L NaCl胁迫下,随着盐浓度的增加,豇豆幼苗叶片可溶性蛋白质、脯氨酸和丙二醛含量逐渐增加,在150mmol/L浓度时,3者的含量都达到最大值;而超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性开始逐渐上升,它们的活性分别在100mmol/L、150mmol/L和150mmol/L时达到最大值,然后逐渐下降。同时,对NaCl胁迫下3种抗氧化酶基因的表达进行适时定量PCR分析,分析结果显示3种抗氧化酶基因的转录表达与酶活性的变化一致。说明在不同浓度的NaCl胁迫下,NaCl诱导了sod、pod和cat3种抗氧化酶基因的表达,3种抗氧化酶活性相应地提高,从而提高了豇豆应对NaCl胁迫的能力。本文结果将为今后豇豆在盐碱栽培生产提供一定的参考。
본연구주요탐토불동농도NaCl협박처리하강두（Vigna unguiculata Linn.）유묘협편항양화매활성적변화정황。연구결과표명,재0～250mmol/L NaCl협박하,수착염농도적증가,강두유묘협편가용성단백질、포안산화병이철함량축점증가,재150mmol/L농도시,3자적함량도체도최대치;이초양화물기화매（SOD）、과양화물매（POD）화과양화경매（CAT）적활성개시축점상승,타문적활성분별재100mmol/L、150mmol/L화150mmol/L시체도최대치,연후축점하강。동시,대NaCl협박하3충항양화매기인적표체진행괄시정량PCR분석,분석결과현시3충항양화매기인적전록표체여매활성적변화일치。설명재불동농도적NaCl협박하,NaCl유도료sod、pod화cat3충항양화매기인적표체,3충항양화매활성상응지제고,종이제고료강두응대NaCl협박적능력。본문결과장위금후강두재염감재배생산제공일정적삼고。
This study focused on the changes of the antioxidant enzymes activities in cowpea seedlings（Vigna unguiculata L.） under different concentrative NaCl stress.The results showed that the content of soluble protein,proline and MDA in cowpea seedlings were gradually increased under 0～250 mmol/L NaCl stress,and their contents were maxmum at 150 mmol/L NaCl.However,the activities of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） were gradually increased and their activities were maximum at 100 mmol/L,150 mmol/L and 150 mmol/L,separately,and the activities falling thereafter.Meanwhile,quantitative real-time PCR analysis of expression of 3 antioxidase genes under NaCl stress also revealed that the over express of 3 antioxidant enzymes were induced by NaCl,and were consistent with the variance of their enzymic activities.Then the increasing of 3 enzymic activities enhanced the tolerance of cowpea against NaCl stress.The results of this paper would be provide a certain theoretical significance for cultivating and producing of cowpea in the future.