CAJ | 학술논문

分析了宁波地区幽门螺杆菌（Helicobacter pylori,Hp）对左氧氟沙星的耐药情况,探讨了Hp对左氧氟沙星的耐药机制.取患者胃黏膜组织分离培养Hp,检测Hp对左氧氟沙星的耐药率;提取左氧氟沙星耐药Hp DNA,PCR扩增并测序比较.结果表明：宁波地区Hp对左氧氟沙星的耐药率为22.5%,左氧氟沙星耐药Hp菌株gyrA基因91位密码子发生点突变.提示宁波地区Hp对左氧氟沙星已产生一定的耐药性;左氧氟沙星耐药Hp gyrA基因突变可能是本地区左氧氟沙星耐药的重要原因之一.
분석료저파지구유문라간균（Helicobacter pylori,Hp）대좌양불사성적내약정황,탐토료Hp대좌양불사성적내약궤제.취환자위점막조직분리배양Hp,검측Hp대좌양불사성적내약솔;제취좌양불사성내약Hp DNA,PCR확증병측서비교.결과표명：저파지구Hp대좌양불사성적내약솔위22.5%,좌양불사성내약Hp균주gyrA기인91위밀마자발생점돌변.제시저파지구Hp대좌양불사성이산생일정적내약성;좌양불사성내약Hp gyrA기인돌변가능시본지구좌양불사성내약적중요원인지일.
In this paper,we examine the antibiotic resistance of Helicobacter pylori（Hp） to levofloxacin in Ningbo,and investigate its possible molecular mechanism of resistance.To determine the resistance rate of Hp to levofloxacin,Hp is detached from mucous membrane of patients’ stomachs,and is subsequently cultured.The levofloxacin-resistant Hp DNA is then extracted,and the gyrA gene is amplified by PCR from resistance and susceptive strains.These PCR-products are sequenced,and the nucleotide sequences are analyzed.The results show that the resistance rate of Hp to levofloxacin is found to be 22.5%,and levofloxacin resistance strains of Hp possesses mutation in codon 91.The Hp strain in Ningbo has developed resistance to levofloxacin,and mutation of gyrA gene plays an important role in the possible mechanism of resistance to levofloxacin.