CAJ | 학술논문

目的:观察清胰化积(Qingyi Huaji,QYHJ)方对人胰腺癌CFPAC-1细胞系裸小鼠移植瘤的抑瘤作用及血清白细胞介素6(interleukin-6,IL-6)、白细胞介素8(interleukin-8,IL-8)和肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达水平的影响.方法:40只裸小鼠右前肢腋部皮下移植胰腺癌瘤块建立荷瘤模型.造模成功的裸小鼠随机分为模型组、卡培他滨组、低剂量(36 g/kg)清胰化积方组和高剂量(72 g/kg)清胰化积方组,每组10只,分别采用含阿拉伯胶的柠檬酸缓冲液、卡培他滨混悬液及清胰化积方药液灌胃.治疗5周后,眼眶取血,酶联免疫吸附测定法检测血清IL-6、IL-8和TNF-α含量;脱颈椎处死裸小鼠,剥取肿瘤,称取质量,测量肿瘤直径,计算抑瘤率.结果:低剂量清胰化积方组CFPAC-1裸小鼠移植瘤质量下降,与模型组比较,差异有统计学意义(P＜0.05).高、低剂量清胰化积方组裸小鼠血清IL-6及TNF-α水平显著低于模型组(P＜0.01),且低剂量清胰化积方组和卡培他滨组血清IL-8水平低于模型组(P＜0.05).相关分析显示,裸小鼠移植瘤质量与血清IL-6、IL-8和TNF-α水平呈线性正相关(P＜0.01).结论:常规剂量的清胰化积方对裸小鼠胰腺癌移植瘤模型有明显的抑瘤作用,其作用机制可能与下调细胞因子IL-6、IL-8和TNF-α的表达有关.
목적:관찰청이화적(Qingyi Huaji,QYHJ)방대인이선암CFPAC-1세포계라소서이식류적억류작용급혈청백세포개소6(interleukin-6,IL-6)、백세포개소8(interleukin-8,IL-8)화종류배사인자α(tumor necrosis factor-α,TNF-α)표체수평적영향.방법:40지라소서우전지액부피하이식이선암류괴건립하류모형.조모성공적라소서수궤분위모형조、잡배타빈조、저제량(36 g/kg)청이화적방조화고제량(72 g/kg)청이화적방조,매조10지,분별채용함아랍백효적저몽산완충액、잡배타빈혼현액급청이화적방약액관위.치료5주후,안광취혈,매련면역흡부측정법검측혈청IL-6、IL-8화TNF-α함량;탈경추처사라소서,박취종류,칭취질량,측량종류직경,계산억류솔.결과:저제량청이화적방조CFPAC-1라소서이식류질량하강,여모형조비교,차이유통계학의의(P＜0.05).고、저제량청이화적방조라소서혈청IL-6급TNF-α수평현저저우모형조(P＜0.01),차저제량청이화적방조화잡배타빈조혈청IL-8수평저우모형조(P＜0.05).상관분석현시,라소서이식류질량여혈청IL-6、IL-8화TNF-α수평정선성정상관(P＜0.01).결론:상규제량적청이화적방대라소서이선암이식류모형유명현적억류작용,기작용궤제가능여하조세포인자IL-6、IL-8화TNF-α적표체유관.
Objective: To investigate the effects of Qingyi Huaji (QYHJ) decoction, a compound traditional Chinese herbal medicine, on tumor inhibition rate and serum levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in nude mice with transplanted tumors of human pancreatic cancer.Methods: The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of pancreatic cancer into the right armpit of 40 BALB/c nude mice. After successful modeling, the mice were randomly divided into untreated group (Arabic gum), capecitabine group, low-dose QYHJ decoction group (36 g/kg) and high-dose QYHJ decoction group (72 g/kg), with 10 mice in each group. Citrate buffer solution (containing 5% Arabic gum), capecitabine suspension and QYHJ decoction were administered to four groups by gavage respectively. After 5-week treatment, the concentrations of serum IL-6, IL-8 and TNF-α were examined by enzyme-linked immunosorbent assay (ELISA) using blood sample from eye socket.Then the mice were euthanized by cervical dislocation. Tumor weight and the tumor inhibition rate were calculated.Results: Tumor weight in the low-dose QYHJ decoction group decreased significantly as compared with the untreated group ( P ＜0.05). Serum levels of IL-6 and TNF-α in low- and high-dose QYHJ groups were extremely significantly lower than those in the untreated group (P＜0.01). Serum level of IL-8 in the low-dose QYHJ group was significantly lower than that in the untreated group (P＜0. 05). Correlation analysis showed that transplanted tumor weight of the mice was linearly positively correlated with serum levels of IL-6, IL-8 or TNF-α (P＜0.01).Conclusion: Conventional dose of QYHJ decoction is effective in suppressing pancreatic carcinoma in nude mice. The mechanism may be related to down-regulation of serum cytokines such as IL-6, IL-8 and TNF-α.