CAJ | 학술논문

[目的]探讨甜菜碱与不同南瓜耐盐性机制之间的关系,明确甜菜碱在耐盐生理中的作用机理.[方法]以黑籽南瓜和青栗南瓜幼苗为试验材料,研究外源甜菜碱(GB)对浓度300 mmol/L NaCl胁迫下2种南瓜幼苗细胞膜透性、MDA和根系活力的影响.渗透率以相对电解质渗透率表示;硫代巴比妥酸比色法测定MDA含量;根系活力采用氯化三苯基四氮唑(TTC)还原法测定;叶绿素含量采用无水乙醇和丙酮(1∶1)混合液提取法.[结果]在合适的浓度下,外源甜菜碱能降低盐胁迫下2种南瓜幼苗叶片质膜透性,抑制膜质过氧化产物MDA的积累,提高植物的根系活力.[结论]甜菜碱处理盐胁迫下的南瓜幼苗能够降低盐胁迫对细胞膜的伤害,缓解盐害,提高南瓜幼苗的耐盐性.
[목적]탐토첨채감여불동남과내염성궤제지간적관계,명학첨채감재내염생리중적작용궤리.[방법]이흑자남과화청률남과유묘위시험재료,연구외원첨채감(GB)대농도300 mmol/L NaCl협박하2충남과유묘세포막투성、MDA화근계활력적영향.삼투솔이상대전해질삼투솔표시;류대파비타산비색법측정MDA함량;근계활력채용록화삼분기사담서(TTC)환원법측정;협록소함량채용무수을순화병동(1∶1)혼합액제취법.[결과]재합괄적농도하,외원첨채감능강저염협박하2충남과유묘협편질막투성,억제막질과양화산물MDA적적루,제고식물적근계활력.[결론]첨채감처리염협박하적남과유묘능구강저염협박대세포막적상해,완해염해,제고남과유묘적내염성.
[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology. [Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials, the effects of glycine betaine on the cell membrane permeability, MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied. [Result] At suitable concentrations, glycine betaine could decrease the leaf plasma-membrane permeability of two varieties of pumpkin seedlings and inhibit the accumulation of lipid peroxide MDA and enhance the root activity of plants. [Conclusion] Pumpkin seedlings under salt stress of glycine betaine could decrease the damage of cell membrane under salt stress, relieve the salt damage and enhance the slat-tolerance of pumpkin seedlings.