CAJ | 학술논문

以陆地棉中35和军棉1号的茎尖为外植体,利用农杆菌介导法将含有拟南芥抗病基因SNC1(suppressor of npr1-1,constitutive 1)转入棉花.对外植体培养时期、农杆菌侵染时间和共培养时间进行改良,实验结果表明,在外植体培养1 d,菌液侵染20 min,并且共培养保持在2 d能够获得较高的遗传转化效率.PCR以及RT-PCR对再生植株T0代和T1代棉花的检测结果表明,SNC1基因已经整合到棉花的基因组中并得到表达.利用浸根法,对T1代转基因棉花接种棉花枯萎病菌强致病力菌株(Fusarium oxysporum f.sp.Vasinfectum),与对照比较,T1代转基因棉花的枯萎病抗性明显提高.
이륙지면중35화군면1호적경첨위외식체,이용농간균개도법장함유의남개항병기인SNC1(suppressor of npr1-1,constitutive 1)전입면화.대외식체배양시기、농간균침염시간화공배양시간진행개량,실험결과표명,재외식체배양1 d,균액침염20 min,병차공배양보지재2 d능구획득교고적유전전화효솔.PCR이급RT-PCR대재생식주T0대화T1대면화적검측결과표명,SNC1기인이경정합도면화적기인조중병득도표체.이용침근법,대T1대전기인면화접충면화고위병균강치병력균주(Fusarium oxysporum f.sp.Vasinfectum),여대조비교,T1대전기인면화적고위병항성명현제고.
Using shoot apex from "Zhong 35" and "Junmian No.1" as explant,we have successfully transferred the disease resistance gene SNC1 (suppressor of nprl-1,constitutive) cloned from A rabiclopsis thaliana into cotton by A grobacterium-mediated transformation.The transformation conditions,including cultural stage of the explant,infection time,and co-culture time,were also optimized.The results showed that the relative high transformation rate was obtained following conditions as follows:one day of explant culture,20 min of infection time,and two days of co-culture.PCR and RT-PCR test of T0 and T1 generations revealed that the SNC1 gene was successfully integrated into cotton and expressed.In addition,T1 generation infected with Fusarium oxysporum f.sp.Vasinfectum through root-dip method were discovered to own improved resistance to the disease compared with non-transgenic plants.