CAJ | 학술논문

目的:通过研究透明质酸寡糖对人肺腺癌细胞系A549/DDP的P糖蛋白和多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)表达的影响,探讨透明质酸在引起肿瘤细胞多药耐药中的作用.方法: 将CD44单抗或透明质酸寡糖与A549/DDP细胞共培养48小时,放免法检测培养基中细胞所分泌透明质酸的含量,流式细胞仪检测经上述处理的A549/DDP表面MDR1 、MRP、LRP的表达率,MTT法检测在不同浓度顺铂作用下,各组细胞的存活率.结果: 经透明质酸寡糖处理后A549/DDP,分泌透明质酸较未处理组明显减少(P<0.05);且细胞表面与多药耐药相关的MDR1 、MRP、LRP的表达率均降低(P<0.05).另外,处理后的细胞,在不同浓度顺铂作用时,细胞凋亡率明显增加.结论: 体外条件下,透明质酸寡糖能逆转A549/DDP的耐药.
목적:통과연구투명질산과당대인폐선암세포계A549/DDP적P당단백화다약내약상관단백(MRP)、폐내약단백(LRP)표체적영향,탐토투명질산재인기종류세포다약내약중적작용.방법: 장CD44단항혹투명질산과당여A549/DDP세포공배양48소시,방면법검측배양기중세포소분비투명질산적함량,류식세포의검측경상술처리적A549/DDP표면MDR1 、MRP、LRP적표체솔,MTT법검측재불동농도순박작용하,각조세포적존활솔.결과: 경투명질산과당처리후A549/DDP,분비투명질산교미처리조명현감소(P<0.05);차세포표면여다약내약상관적MDR1 、MRP、LRP적표체솔균강저(P<0.05).령외,처리후적세포,재불동농도순박작용시,세포조망솔명현증가.결론: 체외조건하,투명질산과당능역전A549/DDP적내약.
Objective:To investigate the effects of hyaluronate oligomers on the multiple drug resistance of lung cancer cell lines A549/DDP. Methods: After co-culturing A549/DDP and CD44 monoclonal antibody or hyaluronan oligomers for 48 hours,to detect the following parameters:Hyaluronate contents of the medium by radioimmunoassay,MDR1,MRP,LRP expressions by flow cytometry,survival rate of cells under different concentrations of cisplatin by MTT tests. Results: Hyaluronan oligomers can decrease hyaluronan expression,and MDR1,MRP,LRP expression of A549/DDP.In addition,apoptosis level of cells treated by hyaluronan oligomers increased significantly in higher concentration cisplatin. Conclution: In vitro,hyaluronan oligomers can reverse drug resistance of A549/DDP.