兰州大学学报（医学版）
蘭州大學學報（醫學版）
란주대학학보（의학판）
JOURNAL OF LANZHOU UNIVERSITY(MEDICAL SCIENCES)
2009年
2期
36-38
,共3页
郗宁%韩俭%吴玲%李宁荫
郗寧%韓儉%吳玲%李寧蔭
치저%한검%오령%리저음
志贺菌%毒力基因%兰州
誌賀菌%毒力基因%蘭州
지하균%독력기인%란주
Shigella%virulent gene%Lanzhou city
目的 调查兰州市分离志贺菌株的毒力基因型.方法 常规分离志贺菌,应用聚合酶链反应检测志贺菌肠毒素1基因(set1A和set1B)、志贺菌肠毒素2基因(sen)、侵袭性质粒抗原H基因(ipaH)、侵袭相关位点基因(ial)和多重调控基因(virA).结果 兰州市志贺菌毒力基因virA、set1A阳性率为100%;ipaH阳性率为93.7%;ial、sell、set1B阳性率分别为56.2%、62.5%、25.0%,set1B仅在福氏志贺菌Ⅳ型中检出.结论 兰州市志贺菌中携带的毒力基因存在差异,virA、set1A和ipaH较稳定,可作为靶基因用于兰州市志贺菌的检验,set1B可用于筛选福氏志贺菌Ⅳ型菌株.
目的 調查蘭州市分離誌賀菌株的毒力基因型.方法 常規分離誌賀菌,應用聚閤酶鏈反應檢測誌賀菌腸毒素1基因(set1A和set1B)、誌賀菌腸毒素2基因(sen)、侵襲性質粒抗原H基因(ipaH)、侵襲相關位點基因(ial)和多重調控基因(virA).結果 蘭州市誌賀菌毒力基因virA、set1A暘性率為100%;ipaH暘性率為93.7%;ial、sell、set1B暘性率分彆為56.2%、62.5%、25.0%,set1B僅在福氏誌賀菌Ⅳ型中檢齣.結論 蘭州市誌賀菌中攜帶的毒力基因存在差異,virA、set1A和ipaH較穩定,可作為靶基因用于蘭州市誌賀菌的檢驗,set1B可用于篩選福氏誌賀菌Ⅳ型菌株.
목적 조사란주시분리지하균주적독력기인형.방법 상규분리지하균,응용취합매련반응검측지하균장독소1기인(set1A화set1B)、지하균장독소2기인(sen)、침습성질립항원H기인(ipaH)、침습상관위점기인(ial)화다중조공기인(virA).결과 란주시지하균독력기인virA、set1A양성솔위100%;ipaH양성솔위93.7%;ial、sell、set1B양성솔분별위56.2%、62.5%、25.0%,set1B부재복씨지하균Ⅳ형중검출.결론 란주시지하균중휴대적독력기인존재차이,virA、set1A화ipaH교은정,가작위파기인용우란주시지하균적검험,set1B가용우사선복씨지하균Ⅳ형균주.
Objective To investigate the virulent genes of Shigella spp separated from the patients from Lanzhou city. Methods Shigella spp was separated from the patients by general methods. The virulent genes of set1A,set1B,sen,ipaH,ial and virA of Shigella spp were detected by PCR. Results The virulent genes of virA and setlA were detected in all Shigella strains. The positive rates of ipaH,ial and sen genes were 93.7%,56.2% and 62.5%,respectively. The positive rate of set1B was 25% and it was only detected in some Shigella flexneri strains. Conclusion There are differences in the virulent genes of Shigella spp separated from the patients from Lanzhou city. VirA,set1A and ipaH present stably in Shigella strains from Lanzhou city and can be used as target genes to detect Shigella. Set1B can be used to screen the type Ⅳ strain of Shigella flexneri.
