CAJ | 학술논문

从患病异育银鲫体内分离到5株可疑菌株,经API 20NE细菌生化鉴定,YCS07-03和YZ07-11株鉴定率为99.2%和99.3%.注射感染中5×108CFU·mL-1剂量组表现出较强的致病性,12 d死亡率100%.菌株在营养肉汤培养液中呈均匀混浊状;在营养琼脂平血中呈灰白色半透明菌落;在血琼脂培养基中呈光滑灰白色菌落,YZ07-11株出现透明的β溶血环;在TCBS培养基呈黄色菌落;在SS和麦康凯培养基中形成无色透明的扁平菌落.经负染电镜观察,菌株两端圆,呈直杆状,无芽孢,极生单鞭毛,菌株大小(短径×长径)分别为1.53 μm×2.71μm和O.80μm×2.1 2μm.菌株经生化实验鉴定为温和气单胞菌(Aeromonas sobria)和嗜水气单胞菌(Aeromonashydrophila).试验结果表明:菌株对丁胺卡那霉素、头孢噻肟、庆大霉素、左氟沙星、复合磺胺、氟嗪酸、洛美沙星、复方新诺明、链霉素和新霉素均产生高度敏感;对万古霉素为中度敏感;氨苄青霉素、羧苄青霉素、强力霉素和青霉素产生耐药.
종환병이육은즉체내분리도5주가의균주,경API 20NE세균생화감정,YCS07-03화YZ07-11주감정솔위99.2%화99.3%.주사감염중5×108CFU·mL-1제량조표현출교강적치병성,12 d사망솔100%.균주재영양육탕배양액중정균균혼탁상;재영양경지평혈중정회백색반투명균락;재혈경지배양기중정광활회백색균락,YZ07-11주출현투명적β용혈배;재TCBS배양기정황색균락;재SS화맥강개배양기중형성무색투명적편평균락.경부염전경관찰,균주량단원,정직간상,무아포,겁생단편모,균주대소(단경×장경)분별위1.53 μm×2.71μm화O.80μm×2.1 2μm.균주경생화실험감정위온화기단포균(Aeromonas sobria)화기수기단포균(Aeromonashydrophila).시험결과표명:균주대정알잡나매소、두포새우、경대매소、좌불사성、복합광알、불진산、락미사성、복방신낙명、련매소화신매소균산생고도민감;대만고매소위중도민감;안변청매소、최변청매소、강력매소화청매소산생내약.