中国科学C辑(英文版)
中國科學C輯(英文版)
중국과학C집(영문판)
SCIENCE IN CHINA (Series C)
2001年
4期
443-448
,共6页
张景强%冯建勋%梁玉尧%谌东华%周正洪%张勤奋%卢炘英
張景彊%馮建勛%樑玉堯%諶東華%週正洪%張勤奮%盧炘英
장경강%풍건훈%량옥요%심동화%주정홍%장근강%로흔영
he RNA of Chinese Sacbrood Bee Virus (CSBV) was purified and used as template to obtain a 1096 bp cDNA fragment by RT-PCR amplification. This DNA fragment was cloned into pGEM-T Easy Vector for sequencing. Analyses of the sequenced CSBV RNA fragment revealed a nucleotide sequence homology of 87.6% and a deduced amino-acid sequence homology of 94.6% with that of the Sacbrood Virus (SBV), indicating that CSBV is a different but highly homologous virus of SBV. The three-dimensional (3D) structure of CSBV was determined at 2.5 nm resolution by using electron cryo-microscopy (cryoEM) and computer reconstruction methods. The 3-D struc-ture showed that the capsid has a T = 1 (or P = 3) icosahedral capsid shell with a smooth surface. There were 12 pentons at its icosahedral vertices (5-fold axes) and 132 holes penetrating the shell. The 3-D structure also revealed densities corresponding to the CSBV genome, suggesting icosa-hedrally-ordered RNA organization, a novel feature not previously reported for any picornaviruses.