CAJ | 학술논문

采用基因枪转化系统，将CpTI（豇豆胰蛋白酶抑制剂）抗虫基因转入水稻愈伤组织中，经过抗生素筛选得到了再生植株。我们用这些转基因水稻再生植株及其后代的分蘖茎段对玉米螟（Ostrinia nubilalis）进行室内喂养，通过比较虫体死亡率及虫体生长长度，测定转基因水稻再生植株的抗虫性。1997～1999年共进行3个世代5次的室内测定。从121株F0代转基因植株中选出抗虫性较好的植株15株。从F1代的15个株系中，又筛选出7个株系40个单株；在F2代的14个株系中共选出37株抗虫效果表现较好的植株。对37株中的19株进行了分子检测（PCR扩增），从中检测出5份材料为纯合体，14份材料有分离现象。研究结果说明：该室内喂养玉米螟并进行抗虫测定的方法是可行的；抗虫基因在转基因水稻再生植株中已经获得表达并且可以遗传
채용기인창전화계통，장CpTI（강두이단백매억제제）항충기인전입수도유상조직중，경과항생소사선득도료재생식주。아문용저사전기인수도재생식주급기후대적분얼경단대옥미명（Ostrinia nubilalis）진행실내위양，통과비교충체사망솔급충체생장장도，측정전기인수도재생식주적항충성。1997～1999년공진행3개세대5차적실내측정。종121주F0대전기인식주중선출항충성교호적식주15주。종F1대적15개주계중，우사선출7개주계40개단주；재F2대적14개주계중공선출37주항충효과표현교호적식주。대37주중적19주진행료분자검측（PCR확증），종중검측출5빈재료위순합체，14빈재료유분리현상。연구결과설명：해실내위양옥미명병진행항충측정적방법시가행적；항충기인재전기인수도재생식주중이경획득표체병차가이유전
Using gene gun transformation system， we transfered CpTI （Cowpea Trypsin Inhibitor）gene to rice callus tissues；through anti-biotic selection.The transgenic regenerative rice plants were gotten． Indoor feeding corn borer（Ostrinia nubilalis） by these transgenic rice regenaration plants and their descendance’s tiller stem；The corn borer mortality and the worm length were compared to determinate transgenic regenerative rice’s pest resistance． From 1997 to 1999，5 indoor tests had been taken on three generations． 15 better pest resistance plants were selected from 121 plants in F0 generation, 7 strains 40 better single plants from 15 strains in F1 generation，and 37 better plants from 14 strains in F2 generation． We did molecular test（PCR） to 19 plants from the 37 better plants，and asserted that 5 material are homozygous plants， the other 14 material have segregation． All of these stated that the anti-pest gene in transgenic regenerative rice can express and is heritable