CAJ | 학술논문

目的　研究新分离的H1N1亚型流感病毒株的HA1基因序列。方法　甲型流感病毒通过鸡胚增殖后提取RNA、逆转录合成cDNA，经PCR扩增和产物纯化构建重组质粒，用双脱氧链终止法进行核苷酸序列测定并进行基因特性分析。结果　新分离到的3株流感病毒株（H1N1）HA1区基因长度为981 bp，编码327个氨基酸；与A／桂防／10／94和A／Bayern／07／95（H1N1）标准株比较其同源性分别为92.8％和91.3％，丢失了第130位氨基酸和304位糖基化位点；新分离的3株甲型流感病毒株（H1N1）HA1区氨基酸同源性高达98％；A／桂防／10／94和A／Bayern ／07／95（H1N1）毒株HN1氨基酸的同源性高达96％。结论　新分离到的3株H1N1毒株HA编码氨基酸不同于A／Bayern／07／95（H1N1）和A／桂防／10／94（H1N1）标准株，它们可能为新的甲型流感病毒变异株。
목적　연구신분리적H1N1아형류감병독주적HA1기인서렬。방법　갑형류감병독통과계배증식후제취RNA、역전록합성cDNA，경PCR확증화산물순화구건중조질립，용쌍탈양련종지법진행핵감산서렬측정병진행기인특성분석。결과　신분리도적3주류감병독주（H1N1）HA1구기인장도위981 bp，편마327개안기산；여A／계방／10／94화A／Bayern／07／95（H1N1）표준주비교기동원성분별위92.8％화91.3％，주실료제130위안기산화304위당기화위점；신분리적3주갑형류감병독주（H1N1）HA1구안기산동원성고체98％；A／계방／10／94화A／Bayern ／07／95（H1N1）독주HN1안기산적동원성고체96％。결론　신분리도적3주H1N1독주HA편마안기산불동우A／Bayern／07／95（H1N1）화A／계방／10／94（H1N1）표준주，타문가능위신적갑형류감병독변이주。
Objective　To obtain genetic characterization of the HA1 of new isolates of influenza A （H1N1） virus. Methods　Virus was amplified in embryonated chicken eggs， the virion DNA was transcribed into cDNA by reverse transcriptase， cDNA amplified by PCR， the products of PCR were purified. Afterward， DNA sequence analysis was performed by the dideoxy-mediated chain termination method， using synthetic oligo nucleotide primers. Results　The HA1 domain of new isolates of influenza A （H1N1） virus showed that their HA1 genes were 981 nucleotide in length coding for a HA1 protein with 327 amino acids， deletion of a glycosylation site and an amino acid. The homology of amino acid sequences of protein molecules on HA1 domains of new influenza A virus when compared with A／Guifang／10／94（H1N1） and A／Bayern ／07／95 （H1N1） viruses，was 92.8％ and 91.3％ respectively； The homology of amino acid sequences of protein molecules on HA1 domains of A／Jingjun／11／98（H1N1） when compared with A／Jingjun／13／98（H1N1） or A／Jingjun／807／97（H1N1） viruses，was as high as 98％； The homology of amino acid sequences of protein molecules on HA1 domains of A／Guifang／10／94（H1N1） when compared with A／Bayern／07／95（H1N1） viruses was as high as 96％. Conclusions　The HA1 gene of　new H1N1 virus strains is　different from those of A／Guifang／10／94 （H1N1） and A／Bayern／07／95（H1N1）， they will probably be new mutation strains.