CAJ | 학술논문

目的探讨端粒酶在胃肠道恶性肿瘤癌组织和癌旁正常组织中的表达及其临床意义。方法应用端粒酶PCR ELISA检测法检测胃癌11例、结肠癌14例及25例癌旁正常组织中的端粒酶活性。结果 25例胃肠道肿瘤组织中21例端粒酶呈阳性(84.0%);25例癌旁正常组织中5例阳性(20.0%)。癌组织和癌旁正常组织中端粒酶的表达均与肿瘤临床病理特征无关。癌旁正常组织存在端粒酶阳性表达的病例,病理上多伴有不典型增生或癌细胞的浸润转移。结论端粒酶可作为实质性肿瘤早期诊断的辅助指标;对癌旁正常组织中端粒酶阳性的患者加强观察,有助于早期发现某些胃肠道恶性肿瘤的术后再发或复发。
목적탐토단립매재위장도악성종류암조직화암방정상조직중적표체급기림상의의。방법응용단립매PCR ELISA검측법검측위암11례、결장암14례급25례암방정상조직중적단립매활성。결과 25례위장도종류조직중21례단립매정양성(84.0%);25례암방정상조직중5례양성(20.0%)。암조직화암방정상조직중단립매적표체균여종류림상병리특정무관。암방정상조직존재단립매양성표체적병례,병리상다반유불전형증생혹암세포적침윤전이。결론단립매가작위실질성종류조기진단적보조지표;대암방정상조직중단립매양성적환자가강관찰,유조우조기발현모사위장도악성종류적술후재발혹복발。
Objective To evaluate the role of telomerase activity in the carcinomas tissue and normal tumor adjacent noncancerous tissues of human gastroenteric tract neoplasms. Methods Telomerase activity in tumor samples,including 11 gastric and 14 colonic, was examined by a novel telomerase PCR ELISA assay. Twenty five samples from normal tumor adjacent tissues were also assayed. Results Twenty one of 25(84.0%) tumor samples and 5 of 25(20.0%) normal adjacent tissue specimens were positive for telomerase activity regardless of tumor size, histological grade and tumor stage. Almost all telomerase positive tumor adjacent non cancerous exist displasia,infiltration and/or metastasis. Conclusion Telomerase may be a good diagnostic biomarker for human gastroenteric tract tumor. The determination of telomerase activity in resected non cancerous tissues may be helpful in predicting postoperative recurrence and/or malignant transformation in human gastroenteric tract neoplasms.