CAJ | 학술논문

目的　建立用HPLC测定血透患者上机前静脉端管路流出液体中微量戊二醛浓度的方法，为临床进一步研究微量戊二醛的来源以及微量戊二醛的清除提供实验依据。方法　取流出液体20 mL,与二硝基苯肼柱前衍生化3 h,过滤，进样20 μL。色谱条件：色谱柱：C18柱；柱温：29℃；流动相：乙腈-磷酸（60∶40）；流速：1 mL.min-1；检测波长：365 nm。结果　血透患者上机前静脉端管路流出液体中存在有微量的戊二醛。结论　HPLC方法能简便，准确地测出血透上机前静脉端管路流出液体中微量戊二醛。
목적　건립용HPLC측정혈투환자상궤전정맥단관로류출액체중미량무이철농도적방법，위림상진일보연구미량무이철적래원이급미량무이철적청제제공실험의거。방법　취류출액체20 mL,여이초기분정주전연생화3 h,과려，진양20 μL。색보조건：색보주：C18주；주온：29℃；류동상：을정-린산（60∶40）；류속：1 mL.min-1；검측파장：365 nm。결과　혈투환자상궤전정맥단관로류출액체중존재유미량적무이철。결론　HPLC방법능간편，준학지측출혈투상궤전정맥단관로류출액체중미량무이철。
OBJECTIVE　To detect the trace glutaraldehyde in the fluid from venous circuit tube of hemodialysis,a HPLC assay was developed.METHODS　20 mL fluid taken from the venous circuit tube were derived with DNPH for 3 h,then filtered and injected. Chromatography was conducted on C18 column at 29℃.The mobile phase was consisted of 60% CH3N and 40% H3PO4 with the flow rate at 1 mL*min-1, and the detetion wavelength was at 365 nm.RESULTS　The concentrations of glutaraldehyde in the fluid ranged from 14.99 to 37.40 μg.mL-1.CONCLUSION　This HPLC method is simple and accurate to detect the trace glutaraldehyde remained in the fluid from venous circuit tube.