中国烟草科学
中國煙草科學
중국연초과학
CHINESE TOBACCO SCIENCE
2001年
1期
37-40
,共4页
烤烟RAPD影响因子优化程序
烤煙RAPD影響因子優化程序
고연RAPD영향인자우화정서
以烤烟品种Hicks和Speight G-28为材料，针对烟草RAPD分析中PCR过程的Taq酶浓度、Mg2+浓度、退火温度、循环数、Pyimer浓度、dNTP浓度等因素进行了研究。结果表明，体系中含0.8 U Taq酶，40mmol/L MgCl2,10μmol/L引物，3.0 mmo1/L dNTP，循环数为40，退火温度36℃效果较好。从而确立适合烤烟RAPD分析的PGR程序主要条件，为RAPD技术应用于烟草的品种鉴定与品种资源研究奠定了基础。
以烤煙品種Hicks和Speight G-28為材料，針對煙草RAPD分析中PCR過程的Taq酶濃度、Mg2+濃度、退火溫度、循環數、Pyimer濃度、dNTP濃度等因素進行瞭研究。結果錶明，體繫中含0.8 U Taq酶，40mmol/L MgCl2,10μmol/L引物，3.0 mmo1/L dNTP，循環數為40，退火溫度36℃效果較好。從而確立適閤烤煙RAPD分析的PGR程序主要條件，為RAPD技術應用于煙草的品種鑒定與品種資源研究奠定瞭基礎。
이고연품충Hicks화Speight G-28위재료，침대연초RAPD분석중PCR과정적Taq매농도、Mg2+농도、퇴화온도、순배수、Pyimer농도、dNTP농도등인소진행료연구。결과표명，체계중함0.8 U Taq매，40mmol/L MgCl2,10μmol/L인물，3.0 mmo1/L dNTP，순배수위40，퇴화온도36℃효과교호。종이학립괄합고연RAPD분석적PGR정서주요조건，위RAPD기술응용우연초적품충감정여품충자원연구전정료기출。
With tobacco variety Hicks and Speight CG-28 as experimental materials, taqase concentration, Mg2+ concentration,annealing temperature,number of amplified cycles,primer concentration and dNTP concenhation of RAPD analysis were studied. The results indicated that under the ccndition of 0.8 U taqase, 40 mmo1/L MgCl2, 10μmol/L primer, 3.0mmol/L dNTP, annealing temperature at 36 ℃C and 40 cycles, the effect was very good. PR APD pattem founded in this experiment layed good foundations for application of BAPD analysis in tobacco variety identification and gennplasm research.
