CAJ | 학술논문

在甲基丙烯酸环氧丙酯(GMA)体外诱导建立人胚肺成纤维细胞(HELF)恶性转化模型的基础上，采用mRNA差异显示PCR(DD-PCR)技术，对恶性转化细胞与正常细胞之间基因的差异表达进行比较分析。结果显示，两种细胞在基因表达方面存在明显差异。已克隆到的3个差异表达的基因片段分别与人乳腺癌转录因子(ZABC1)、Tbx3和小鼠E2基因高度同源，并在转化细胞中均呈明显的上调表达。结果提示，包括ZABC1、Tbx3和E2在内的多个基因的激活与失活可能与GMA诱导的细胞恶性转化过程有关。
재갑기병희산배양병지(GMA)체외유도건립인배폐성섬유세포(HELF)악성전화모형적기출상，채용mRNA차이현시PCR(DD-PCR)기술，대악성전화세포여정상세포지간기인적차이표체진행비교분석。결과현시，량충세포재기인표체방면존재명현차이。이극륭도적3개차이표체적기인편단분별여인유선암전록인자(ZABC1)、Tbx3화소서E2기인고도동원，병재전화세포중균정명현적상조표체。결과제시，포괄ZABC1、Tbx3화E2재내적다개기인적격활여실활가능여GMA유도적세포악성전화과정유관。
The malignant transformation of human embryonic lungfibroblasts(HELF)induced by glycidyl methacrylate(GMA) in vitro was used as a model for comparing gene expression between the transformed cells and controls.Using mRNA differential display PCR(DD-PCR) technique, the three cDNA fragments up-regulated in the transformed cells were identified and highly homologous to human breast cancer transcription factor(ZABC1),Tbx3 and mouse ubiquitin conjugating enzyme(E2) gene.These results suggest that activation or inactivation of genes including ZABC1,Tbx3 and E2 may be involved in GMA-induced transformation.