CAJ | 학술논문

目的：探讨肾移植受者外周血T淋巴细胞亚群与发生重症巨细胞病毒(CMV)感染和急性排斥反应(AR)之间的关系。方法：采用COULTER公司生产的鼠抗人T淋巴细胞亚群单克隆抗体OKT系列及ELITE型流式细胞仪对23例重症CMV感染者、8例AR患者和20例正常肾移植者的外周血T淋巴细胞亚群进行动态测定。结果：术后无AR者与AR者、排斥反应缓解者与未缓解者，其CD4/CD8比值变化差异有显著性意义(P＜0.05)；而发生重症CMV感染者的CD4/CD8比值变化与AR者完全相反。结论：外周血T淋巴细胞亚群的动态变化对肾移植受者AR和重症CMV感染的诊断、鉴别诊断以及指导临床合理用药有一定意义。
목적：탐토신이식수자외주혈T림파세포아군여발생중증거세포병독(CMV)감염화급성배척반응(AR)지간적관계。방법：채용COULTER공사생산적서항인T림파세포아군단극륭항체OKT계렬급ELITE형류식세포의대23례중증CMV감염자、8례AR환자화20례정상신이식자적외주혈T림파세포아군진행동태측정。결과：술후무AR자여AR자、배척반응완해자여미완해자，기CD4/CD8비치변화차이유현저성의의(P＜0.05)；이발생중증CMV감염자적CD4/CD8비치변화여AR자완전상반。결론：외주혈T림파세포아군적동태변화대신이식수자AR화중증CMV감염적진단、감별진단이급지도림상합리용약유일정의의。
Purpose :To investigate the relationship of peripheral blood T-lymphocyte subsets with the acuterejection or severe CMV infection in transplanted patients. Methods :T-lymphocytes subsets of peripheral bloodwere consecutively detected by using mice-verse-human T-lymphocytes subsets monoclonal antibody-OKT serialsand flow cytometer. Results:The difference of CD4/CD8 ratios between the no acute rejection group and the acuterejection group, or between the acute rejection remission group and the resistant acute rejection group was signif-icant ( P ＜0.05); In patients with intensive CMV infection, the CD4/CD8 ratios were converse to the acute re-jection group. Conclusions:These results indicated that monitoring of peripheral blood T-lymphocyte subsets wasof much benefit to early diagnosis and differential diagnosis of acute rejection of intensive CMV infection and rea-sonable treatment.