CAJ | 학술논문

目的：观察白细胞介素-2(IL-2)基因转导后CD3AK细胞免疫学功能的变化。方法：应用逆转录病毒载体将IL2基因转导入CD3AK细胞。检测转导细胞中特异性NeoR基因、培养上清IL2的表达水平及转导CD3AK细胞的体外增殖活性、细胞毒活性和细胞表型。结果：从转导细胞mRNA中扩增出长度为347bp的特异性NeoR基因片段，转导细胞培养上清的IL2表达水平显著增高，体外增殖活性和细胞毒活性均强于未转导组细胞，CD4+/CD2+值升高。结论：PLIL2SN逆转录病毒转导CD3AK细胞后，IL-2基因得到表达并增强CD3AK细胞的免疫学功能。
목적：관찰백세포개소-2(IL-2)기인전도후CD3AK세포면역학공능적변화。방법：응용역전록병독재체장IL2기인전도입CD3AK세포。검측전도세포중특이성NeoR기인、배양상청IL2적표체수평급전도CD3AK세포적체외증식활성、세포독활성화세포표형。결과：종전도세포mRNA중확증출장도위347bp적특이성NeoR기인편단，전도세포배양상청적IL2표체수평현저증고，체외증식활성화세포독활성균강우미전도조세포，CD4+/CD2+치승고。결론：PLIL2SN역전록병독전도CD3AK세포후，IL-2기인득도표체병증강CD3AK세포적면역학공능。
Objective This experiment was designed to observe the immunologic functions of CD3AK cells into which interleukin-2(IL-2) gene had been transducted. Methods The post-transfer CD3AK cells' special NeoR gene and cell immunologic functions including IL-2 expression, proliferation, cytotoxicity and cell phonetype were detected. R~ults The specific 347 bp NeoR gene was amplfiied in post-transfer cells. The post-transfer cells expressed higher IL-2, proliferation and cytotoxicity ability. It was also found that the ratio of CD4 + T cell to CDa + T cell increased in post-transfer group. Conclusion Transducting IL-2 gene into CD3AK cells could enhance their immunologic functions.