CAJ | 학술논문

由海产贝类中分离到一菌株YS-9412-130，为革兰氏阴性短杆菌、末端圆、有鞭毛、不能滑行运动、大小为0.4～0.7μm×1.0～1.5μm，裂殖生殖，不生成芽孢，胞内无多-β-羟基丁酸颗粒。菌落光滑，黄色、半透明，边缘完整。其色素无荧光，含类胡萝卜素，其正己烷提取液经光谱测定最大吸收峰为450nm。该菌生长的温度范围为0～25℃，最适为18～22℃，最高25℃(在25℃生长极弱或几乎不生长)。此菌株于pH7.0～9.0能生长，最适pH为7.5，耐6％NaCl，80μg／m1的链霉素或80μg/ml的氨卞青霉素。YS-9412-130菌株为好气菌，严格的有氧呼吸代谢,过氧化氢酶、氧化酶阳性和磷酸酶阳性,利用多种糖、水解淀粉但不分解纤维素、琼脂和几丁质。水解干酪素、胨化石蕊牛奶最后变碱。液化明胶试验阴性，还原硝酸盐；不产H2S和吲哚；MR和V．P．试验阴性；YS-9412-130菌株DNA中G+C含量为32.2mol％。该菌株的特征符合黄杆菌属的特征，但与此属中的其他种比较又有显著的差别，因此将其定为黄杆菌属一新种，命名为黄海黄杆菌（Flavobacterium yellowsea sp.nov）。
유해산패류중분리도일균주YS-9412-130，위혁란씨음성단간균、말단원、유편모、불능활행운동、대소위0.4～0.7μm×1.0～1.5μm，렬식생식，불생성아포，포내무다-β-간기정산과립。균락광활，황색、반투명，변연완정。기색소무형광，함류호라복소，기정기완제취액경광보측정최대흡수봉위450nm。해균생장적온도범위위0～25℃，최괄위18～22℃，최고25℃(재25℃생장겁약혹궤호불생장)。차균주우pH7.0～9.0능생장，최괄pH위7.5，내6％NaCl，80μg／m1적련매소혹80μg/ml적안변청매소。YS-9412-130균주위호기균，엄격적유양호흡대사,과양화경매、양화매양성화린산매양성,이용다충당、수해정분단불분해섬유소、경지화궤정질。수해간락소、동화석예우내최후변감。액화명효시험음성，환원초산염；불산H2S화신타；MR화V．P．시험음성；YS-9412-130균주DNA중G+C함량위32.2mol％。해균주적특정부합황간균속적특정，단여차속중적기타충비교우유현저적차별，인차장기정위황간균속일신충，명명위황해황간균（Flavobacterium yellowsea sp.nov）。
The strain YS-9412-130 is isolated from marine shells. The cells of strain are short rods with rounded ends, measuring 0.4～0.7μm by 1.0～1.5μm, gram-positive, flagellated, not gliding movement, reproduction by schizogenesis. Endospores not formed. Intracellular granules of poly-β-hydroxybutyrate are absent. Colonies of strain YS-9412-130 are yellow colour, translucent and smooth with entire edges. The pigments are non-fluorescent, and include carotenoids. The maximum absorption peak of the pigments in n-hexane is 450nm.The temperature range for growth is 0 to 25℃, minimum, 5℃, optimum, 18 to 22℃; maximum, 25℃(very week growth or almost no growth ); the pH range for growth is 7.0～9.0, optimum growth occurred at pH7.5. This strain may be tolerant of 6%NaCl, 80μg/ml of streptomycin and of ampicillin. 　　Strain YS-9412-130 is aerobic, having a strictly respiratory type of metabolism. Catalase-,oxidase- and phosphatase-positive. Many sugars are utilized and alkalified. It hydrolyzes starch, but cellulose (CMC or filter paper), chitin and agar are not digested. This strain hydrolyzes casein, and peptonizes litmus milk and turns it into alkaline. Gelatin isnt liquefied. Nitrate is reduced to nitrite. M.R. and V.P. tests are negative. H2S and indole are not produced. The guanine plus cytosine content of deoxyribonucleic acid of the strain YS-9412-130 is 32.2mol%. This strain belongs to the genus Flavobacterium. When it is compared with descriptions of Flavobacterium,it differs significantly from strains of previously described species. Therefore, it is regarded as a new species. According to its character of growth, the name Flavobacterium yellowsea sp.nov is proposed.