CAJ | 학술논문

目的研究放射性核素188铼(188Re)诱导乳腺癌 ER-75-30细胞凋亡及其与bcl-2和bax基因表达的关系. 方法应用光镜、电镜、流式细胞仪和免疫组化方法检测不同浓度 188Re作用于体外培养的乳腺癌ER-75-30细胞后,诱导细胞凋亡及bcl-2和bax基因表达情况。结果188Re以诱导乳腺癌ER-75-30细胞发生凋亡形态学变化，并且随着188Re浓度增大，凋谢亡率增加，bcl-2表达减弱，bax表达增强。结论188Re能诱导乳腺癌ER-75-30细胞凋谢亡且具有剂量和周期依赖性，bcl-2和bax基因在188Re诱导的细胞凋亡过程中具有重要作用。
목적연구방사성핵소188래(188Re)유도유선암 ER-75-30세포조망급기여bcl-2화bax기인표체적관계. 방법응용광경、전경、류식세포의화면역조화방법검측불동농도 188Re작용우체외배양적유선암ER-75-30세포후,유도세포조망급bcl-2화bax기인표체정황。결과188Re이유도유선암ER-75-30세포발생조망형태학변화，병차수착188Re농도증대，조사망솔증가，bcl-2표체감약，bax표체증강。결론188Re능유도유선암ER-75-30세포조사망차구유제량화주기의뢰성，bcl-2화bax기인재188Re유도적세포조망과정중구유중요작용。
AIM To study apoptosis of human breast cancer ER-75-30 cell induced by 188Re and expression of bcl-2 gene and bax gene. METHODS Light microscope, transmissional electron microscope, flow cytometer and immunohistochemical method were used to observed ER-75-30 cells apoptosis after expose to 188Re of different doses and expressing of bcl-2 and bax. RESULTS 188Re can induced ER-75-30 cell producing typical morphologic changes of apoptosis and with the rise of radiation dose, cell apoptosis rate increased, bcl-2 gene decreased and bax gene was enhanced. Cells were blocked in G2/M period. CONCLUSION Radionuclide 188Re can induce tumor cell apoptosis. This effect takes on dose-effect relation and cellcycle dependent. bcl-2 and bax gene play import part in the course.