CAJ | 학술논문

以牛分枝杆菌(Mycobacterium bovis)Valleelll株染色体DNA为模板,以RecA基因特异性引物进行PCR扩增,获得约240 bp的DNA片段.将PCR产物克隆至pMD~(TM)18-T Vector中,通过α-互补法筛选、质粒PCR鉴定及序列分析鉴定,成功构建出了重组质粒pMD~(TM)18-T-RecA,为进一步研究RecA基因及其在牛结核病诊断中的应用奠定了基础.
이우분지간균(Mycobacterium bovis)Valleelll주염색체DNA위모판,이RecA기인특이성인물진행PCR확증,획득약240 bp적DNA편단.장PCR산물극륭지pMD~(TM)18-T Vector중,통과α-호보법사선、질립PCR감정급서렬분석감정,성공구건출료중조질립pMD~(TM)18-T-RecA,위진일보연구RecA기인급기재우결핵병진단중적응용전정료기출.
The chromosome DNA was extracted from Mycobacterium bovis Valleelll,the RecA gene was amplified with a pair of specific primers by using polymerase chain reaction (PCR). The PCR product was approximately 240 bp DNA segment. The PCR product was cloned into pMD?8-T Vector,then by using a-complementation test,PCR assay and recom-binant plasmid sequential analysis,recombinant plasmid pMD?8-T-RecA was successfully constructed. These results could serve as a solid basis for further studies on the usefulness of RecA gene and its application in diagnosis of tuberculosis.