CAJ | 학술논문

目的:探讨敲低PI3Kp85α表达对人乳腺癌细胞系MCF-7细胞生长的影响和机制.方法:用靶向PI3Kp85α的siRNA转染人乳腺癌细胞系MCF-7,使用Real-time PCR法鉴定转染PI3Kp85α表达水平;MTT法评价PI3Kp85α siRNA对乳腺癌细胞系MCF-7生长的影响;流式细胞术检测转染后细胞周期分布和凋亡;采用免疫荧光染色及Western blot方法观察IA型PI3K/AKT通路主要成员的表达.结果:Real-timePCR结果显示PI3Kp85α siR-NA转染导致PI3Kp85α表达下调;MTT结果显示PI3Kp85α siRNA转染抑制肿瘤细胞生长;流式细胞术检测可见PI3Kp85α siRNA转染组细胞周期存在G_0/G_1期阻滞而且凋亡率显著高于对照组与空载体组(F=19.255,P=0.002).结论:应用PI3Kp85α siRNA转染人乳腺癌细胞系MCF-7细胞,可抑制其增殖和诱导细胞凋亡,因此PI3Kp85α可以作为人乳腺癌基因治疗的候选靶点.
목적:탐토고저PI3Kp85α표체대인유선암세포계MCF-7세포생장적영향화궤제.방법:용파향PI3Kp85α적siRNA전염인유선암세포계MCF-7,사용Real-time PCR법감정전염PI3Kp85α표체수평;MTT법평개PI3Kp85α siRNA대유선암세포계MCF-7생장적영향;류식세포술검측전염후세포주기분포화조망;채용면역형광염색급Western blot방법관찰IA형PI3K/AKT통로주요성원적표체.결과:Real-timePCR결과현시PI3Kp85α siR-NA전염도치PI3Kp85α표체하조;MTT결과현시PI3Kp85α siRNA전염억제종류세포생장;류식세포술검측가견PI3Kp85α siRNA전염조세포주기존재G_0/G_1기조체이차조망솔현저고우대조조여공재체조(F=19.255,P=0.002).결론:응용PI3Kp85α siRNA전염인유선암세포계MCF-7세포,가억제기증식화유도세포조망,인차PI3Kp85α가이작위인유선암기인치료적후선파점.
Objective: To study the effect of and possible mechanism of knockinng down PI3Kp85α using siRNA in MCF-7 human breast cancer cell line. Methods: Oligofectamine was used to transfect PI3Kp85α siRNA to knock down the PI3Kp85α expression level in MCF-7 human breast cancer cell line in vitro. Real-time PCR was conducted to detect the expression of PI3Kp85α. The effect of PI3Kp85αsiRNA on the growth of MCF-7 cells was measured by MTT. The cell cycle distribution and cell apoptosis were detected by cell flow cytometry. Protein expression was evaluated by immunofluorescence staining and Western blot. Results: The expression of PI3Kp85 α was knocked down with PI3Kp85α siRNA in MCF-7 cells. Cell growth was delayed in PI3Kp85αsiRNA-treated group. Conclusion: The suppressive effect of PI3Kp85αsiRNA on the growth of MCF-7 human breast cancer cell line is significant and PI3Kp85α could be a candidate for gene therapy for breast cancer.