CAJ | 학술논문

利用Genbank中大西洋鳕的7对微卫星引物,对太平洋鳕基因组DNA进行PCR扩增.这7对引物均可较稳定地扩增出条带,它们分别是Gmo2、Gmo3、Gmo8、Gmo19、Gmo35、Gmo36、Gmo37,并且扩增出的7个位点均表现出多态性,共获得69个等位基因,每个位点的等位基因数目为4～17,大小为111～227 bp.在7个位点上2种鱼的等位基因的大小存在着不同程度的差异.其中Gmo37在大西洋鳕和太平洋鳕中扩增出的条带的长度差异最大,基本无重合部分,分别为220～290 bp和144～226 bp,本研究结果以期对2种鳕科鱼类遗传结构的进一步研究提供帮助.
이용Genbank중대서양설적7대미위성인물,대태평양설기인조DNA진행PCR확증.저7대인물균가교은정지확증출조대,타문분별시Gmo2、Gmo3、Gmo8、Gmo19、Gmo35、Gmo36、Gmo37,병차확증출적7개위점균표현출다태성,공획득69개등위기인,매개위점적등위기인수목위4～17,대소위111～227 bp.재7개위점상2충어적등위기인적대소존재착불동정도적차이.기중Gmo37재대서양설화태평양설중확증출적조대적장도차이최대,기본무중합부분,분별위220～290 bp화144～226 bp,본연구결과이기대2충설과어류유전결구적진일보연구제공방조.
Seven microsatellite primers in Atlantic cod (Gadus morhua) from Genebank were used to sequence the DNA genes in Pacific cod (G. Macrocephalus ) by polymerase chain reactions (PCRs) and showed stably strips named as Gmo2,Gmo3,Gmo8,Gmo19,Gmo35,Gmo36,and Gmo37. Seven loci were found to be polymorphism. The result showed 69 alleles with the number of 4～17 in every allele of each locus with 111～227 bp in size. There were differences with various degree between the two cods. For example,the strip of Gmo37 was the most different in the size of the fragments in Pacific cod and Atlantic cod,without superposition parts,220～290 bp and 144～226 bp.