CAJ | 학술논문

目的观察低氧下丹参酮ⅡA(Tan ⅡA)对人胃癌SGC7901细胞c-Met蛋白表达的影响及其与低氧诱导因子-1α(HIF-1α)表达的关系.方法用氯化钴(CoCl2)创建低氧模型,设常氧对照组、低氧对照组和低氧加不同浓度Tan ⅡA组.分别取0.5、2.0、10.0mg/L Tan ⅡA干预低氧胃癌细胞48 h后,免疫细胞化学二步法检测HIF-1α和c-Met蛋白表达的变化.结果免疫细胞化学法显示,TanⅡA呈剂量依赖性地抑制低氧诱导的HIF-1α和c-Met蛋白表达,且二者呈高度正相关(n=4,r=0.996,P<0.01).结论低氧条件下,Tan ⅡA可能通过抑制HIF-1α的表达进而抑制c-Met蛋白的表达,提示Tan ⅡA可能对防治低氧导致的肿瘤侵袭转移具有重要作用.
목적 관찰저양하단삼동ⅡA(Tan ⅡA)대인위암SGC7901세포c-Met단백표체적영향급기여저양유도인자-1α(HIF-1α)표체적관계.방법 용록화고(CoCl2)창건저양모형,설상양대조조、저양대조조화저양가불동농도Tan ⅡA조.분별취0.5、2.0、10.0mg/L Tan ⅡA간예저양위암세포48 h후,면역세포화학이보법검측HIF-1α화c-Met단백표체적변화.결과 면역세포화학법현시,TanⅡA정제량의뢰성지억제저양유도적HIF-1α화c-Met단백표체,차이자정고도정상관(n=4,r=0.996,P<0.01).결론 저양조건하,Tan ⅡA가능통과억제HIF-1α적표체진이억제c-Met단백적표체,제시Tan ⅡA가능대방치저양도치적종류침습전이구유중요작용.
Objective To study the effects of Tan Ⅱ A on the expression of c-Met and HIF-lα in gastric cancer SGC7901 cells under hypoxia and the correlation between c-Met and HIF-lα expression. Methods The model of hypoxia was established by CoCl2. There were three groups; normal control group, hypoxia control group, and hypoxia combined with different concentrations of Tan ⅡA group. After Tan ⅡA was added to the media with 0.5, 2.0 and 10.0 mg/L respectively for 48 h under hypoxia, the expression of HIF-1α and c-Met protein was detected by immunocytochemical staining. Results Immunocytochemical staining revealed that the expression of HIF-1α and c-Met protein was inhibited by Tan ⅡA in a dose-dependent manner under hypoxia. High positive correlation was found between the expression of HIF-1α and c-Met (n = 4, r = 0. 996, P <0.01). Conclusion Tan ⅡA might inhibit the expression of c-Met protein via down-regulation of HIFlα protein expression under hypoxia, suggesting that Tan ⅡA might play an important role in fighting against hypoxia-stimulated invasion and metastasis of tumor.