CAJ | 학술논문

目的研究观察复方中药提取物(葆盛口服液)对体外培养的人宫颈癌Hela细胞生长的影响及其作用机制.方法以人宫颈癌Hela细胞为研究对象.用MTT法观察葆盛口服液对Hela细胞生长的影响,以肝细胞株7701作为比较;流式细胞仪分析Hela细胞凋亡以及凋亡基因Fas的表达情况,以顺铂作为对照.结果葆盛口服液对Hela细胞有较强的体外增殖抑制作用,流式细胞术发现葆盛口服液能促进Fas表达,诱导Hela细胞凋亡.结论葆盛口服液诱导细胞凋亡,在抗肿瘤作用中可能具有重要意义.
목적 연구관찰복방중약제취물(보성구복액)대체외배양적인궁경암Hela세포생장적영향급기작용궤제.방법 이인궁경암Hela세포위연구대상.용MTT법관찰보성구복액대Hela세포생장적영향,이간세포주7701작위비교;류식세포의분석Hela세포조망이급조망기인Fas적표체정황,이순박작위대조.결과 보성구복액대Hela세포유교강적체외증식억제작용,류식세포술발현보성구복액능촉진Fas표체,유도Hela세포조망.결론 보성구복액유도세포조망,재항종류작용중가능구유중요의의.
Objective To investigate the influence of the traditional Chinese medicine extract (Baosheng Oral Liquid) on the growth of human cell line Hela in vitro and its probable mechanism, Methods The inhibition of Baosheng Oral Liquid on Hela cells and human normal liver line 7701 cells proliferation was detected by MTT method, cell apoptosis and expression of apoptosis gene fas were further analyzed with flow cytometry in which anti-tumor drug Cisplatin was employed as control. Results Baosheng Oral Liquid remarkably inhibited the growth of Hela cells. Flow cytometric analysis showed that apoptosis of the tumor cells was induced and expression of Fas was enhanced. Conclusion apoptosis may play an important role in the anti-tumor effect of Baosheng Oral Liquid.