中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
2期
173-177
,共5页
石结武%周林玉%谈宜傲%古爱军%于靳%宣强
石結武%週林玉%談宜傲%古愛軍%于靳%宣彊
석결무%주림옥%담의오%고애군%우근%선강
肾细胞癌%趋化因子受体4%血管内皮生长因子%免疫组化
腎細胞癌%趨化因子受體4%血管內皮生長因子%免疫組化
신세포암%추화인자수체4%혈관내피생장인자%면역조화
Renal cell carcinoma%Chemokine receptor-4%Vascular endothelial growth factor%Immunohistochemistry
目的 探讨趋化因子受体4(CXCR4)和血管内皮生长因子(VEGF)在肾癌的发生、发展中的作用及临床意义.方法 采用免疫组织化学技术SP法检测CXCR4和VEGF在56例肾癌标本(包括20例伴有淋巴结转移的肾癌组织及肾周转移的淋巴结)、10例癌旁正常组织中的表达.结果 56例肾细胞癌组织中CXCR4、VEGF的表达阳性率分别为66.1%(37/56),73.2%(41/56),明显高于正常肾组织的表达阳性率20.0%(2/10)、30.0%(3/10),两者间差异有统计学意义(P＜0.05＝.肾癌组织中CXCR4和VEGF的表达呈正相关(r=0.315,P＜0.05＝,CXCR4和VEGF的表达与肾细胞癌的分期(χ2=9.520,P=0.023;χ2=9.072,P=0.027),肿瘤的侵袭转移(χ2=4.972,P=0.026;χ2=3.910,P=0.034)及微血管密度(P＜0.05＝有关,与患者的性别(χ2=0.020,P=0.887;χ2=0.001,P=0.716)、肿瘤的大小(χ2=0.003,P=0.995;χ2=0.108,P=0.990)、病理类型(χ2=1.960,P=0.900;χ2=0.112,P=0.994)无关.结论 CXCR4和VEGF在肾癌中呈高表达并且表达呈正相关,与肾癌的发展转移及预后密切相关.可以作为判断肾癌的侵袭转移及预后的重要指标,并给肾癌的治疗提供前景.
目的 探討趨化因子受體4(CXCR4)和血管內皮生長因子(VEGF)在腎癌的髮生、髮展中的作用及臨床意義.方法 採用免疫組織化學技術SP法檢測CXCR4和VEGF在56例腎癌標本(包括20例伴有淋巴結轉移的腎癌組織及腎週轉移的淋巴結)、10例癌徬正常組織中的錶達.結果 56例腎細胞癌組織中CXCR4、VEGF的錶達暘性率分彆為66.1%(37/56),73.2%(41/56),明顯高于正常腎組織的錶達暘性率20.0%(2/10)、30.0%(3/10),兩者間差異有統計學意義(P＜0.05＝.腎癌組織中CXCR4和VEGF的錶達呈正相關(r=0.315,P＜0.05＝,CXCR4和VEGF的錶達與腎細胞癌的分期(χ2=9.520,P=0.023;χ2=9.072,P=0.027),腫瘤的侵襲轉移(χ2=4.972,P=0.026;χ2=3.910,P=0.034)及微血管密度(P＜0.05＝有關,與患者的性彆(χ2=0.020,P=0.887;χ2=0.001,P=0.716)、腫瘤的大小(χ2=0.003,P=0.995;χ2=0.108,P=0.990)、病理類型(χ2=1.960,P=0.900;χ2=0.112,P=0.994)無關.結論 CXCR4和VEGF在腎癌中呈高錶達併且錶達呈正相關,與腎癌的髮展轉移及預後密切相關.可以作為判斷腎癌的侵襲轉移及預後的重要指標,併給腎癌的治療提供前景.
목적 탐토추화인자수체4(CXCR4)화혈관내피생장인자(VEGF)재신암적발생、발전중적작용급림상의의.방법 채용면역조직화학기술SP법검측CXCR4화VEGF재56례신암표본(포괄20례반유림파결전이적신암조직급신주전이적림파결)、10례암방정상조직중적표체.결과 56례신세포암조직중CXCR4、VEGF적표체양성솔분별위66.1%(37/56),73.2%(41/56),명현고우정상신조직적표체양성솔20.0%(2/10)、30.0%(3/10),량자간차이유통계학의의(P＜0.05＝.신암조직중CXCR4화VEGF적표체정정상관(r=0.315,P＜0.05＝,CXCR4화VEGF적표체여신세포암적분기(χ2=9.520,P=0.023;χ2=9.072,P=0.027),종류적침습전이(χ2=4.972,P=0.026;χ2=3.910,P=0.034)급미혈관밀도(P＜0.05＝유관,여환자적성별(χ2=0.020,P=0.887;χ2=0.001,P=0.716)、종류적대소(χ2=0.003,P=0.995;χ2=0.108,P=0.990)、병리류형(χ2=1.960,P=0.900;χ2=0.112,P=0.994)무관.결론 CXCR4화VEGF재신암중정고표체병차표체정정상관,여신암적발전전이급예후밀절상관.가이작위판단신암적침습전이급예후적중요지표,병급신암적치료제공전경.
Objective To study the role and clinical significance of chemokine receptor-4 (CXCR4) and vascular endothelial growth factor (VEGF) in the occurrence and development of renal cell carcinoma. Methods Expression of CXCR4 and VEGF were detected by SP immunohistochemical technique in 56 cases of kidney carcinoma tissues (including 20 cases of lymph node metastasis), 10 normal tissues nearby kidney cancer. Results The positive rates of CXCR4 and VEGF were 66. 1% (37/56) and 73. 2% (41/56),which were significantly higher than those in normal tissues( 20. 0% (2/10) and 30. 0% (3/10), respectively) (P ＜ 0. 05 ＝. The expression of CXCR4 protein was significantly positively correlated with that of VEGF protein (r = 0. 315 ,P ＜ 0.05 ＝ in renal cell carcinoma. The expression of CXCR4 and VEGF was closely related to stages of tumor ( χ2 = 9. 520, P = 0. 023; χ2 = 9. 072, P = 0. 027 ), lymphatic metastasis, degree of invasion ( χ2 =4. 972, P = 0. 026; χ2 = 3.910, P = 0. 034 ), and microvessel density ( MVD) ( P ＜ 0. 05 ＝. However, they were not related to sex ( χ2 = 0. 020, P= 0. 887; χ2 = 0. 001, P = 0. 716 ), tumor size ( χ2 = 0. 003, P = 0. 995; χ2 =0. 108, P = 0. 990) and pathologic types ( χ2 = 1. 960, P = 0. 900; χ2 = 0. 112, P = 0. 994). Conclusion There is a significant positive correlation between high expressions of CXCR4 and VEGF proteins in renal cell carcinoma,the high expressions of CXCR4 and VEGF proteins may be related to the metastasis and prognosis of renal cell carcinoma,thus they could be used as important indicators in judging the metastasis prognosis of renal cell carcinoma,and offer prospects for the treatment of renal cell carcinona.
