CAJ | 학술논문

目的观察两种不同保存方法对微小隐孢子虫卵囊感染小鼠能力的影响.方法用PBS稀释纯化的卵囊,加入二甲基亚砜(DMSO),置-40℃ 3h后,放入液氮中保存;或加入2.5%重铬酸钾中,4℃保存.3个月后取出,观察它们的脱囊率;分别感染用地塞米松免疫抑制后的BALB/c小鼠,计数卵囊排出量,计算平均每克粪便中的卵囊数目,同时记录两组小鼠的死亡时间.分别采用t检验和成组设计的两样本秩和检验进行统计学分析.结果液氮中保存3个月的卵囊能够感染免疫抑制的BALB/c小鼠,其脱囊率为7.2%～49.6%、卵囊排出量为(4.7～7.3)×10~5/g,接种后实验小鼠死亡时间为124～351 h,与4℃保存的卵囊差别无统计学意义[8.3%～51.2%,(4.6～6.9)×10~5/g,132～348 h,P均>0.05)].结论液氮中保存3个月的卵囊与4℃保存的卵囊对免疫抑制的小鼠具有同样的感染力,突破了微小隐孢子虫卵囊只能在4℃保存的传统观念,为隐孢子虫卵囊的长期保存提供了理论和实践依据.
목적 관찰량충불동보존방법 대미소은포자충란낭감염소서능력적영향.방법 용PBS희석순화적란낭,가입이갑기아풍(DMSO),치-40℃ 3h후,방입액담중보존;혹가입2.5%중락산갑중,4℃보존.3개월후취출,관찰타문적탈낭솔;분별감염용지새미송면역억제후적BALB/c소서,계수란낭배출량,계산평균매극분편중적란낭수목,동시기록량조소서적사망시간.분별채용t검험화성조설계적량양본질화검험진행통계학분석.결과액담중보존3개월적란낭능구감염면역억제적BALB/c소서,기탈낭솔위7.2%～49.6%、란낭배출량위(4.7～7.3)×10~5/g,접충후실험소서사망시간위124～351 h,여4℃보존적란낭차별무통계학의의[8.3%～51.2%,(4.6～6.9)×10~5/g,132～348 h,P균>0.05)].결론 액담중보존3개월적란낭여4℃보존적란낭대면역억제적소서구유동양적감염력,돌파료미소은포자충란낭지능재4℃보존적전통관념,위은포자충란낭적장기보존제공료이론화실천의거.
Objective To observe the infectivity of Cryptosporidium porvum oocysts preserved in two different ways.Methods Purified oocysts were stored at 4℃in 2.5%potassium dichromate or preserved in liquid nitrogen in the presence of cryoprotectant dimethyl sulfoxide(DMSO)for 3 months,then inoculated to BALB/c immuno-depressed mice respectively.The number of oocysts per gram feces and the survival time of infected mice after inoculation in two groups were analyzed by t test and rank sum test respectively.Results Both the purified oocysts kept in liquid nitrogen or stored at 4℃in 2.5%potassium dichromate exhibited infectivity to mice.There were no significant differences in two groups in terms of excystation rate(7.2%-49.6%,8.3%-51.2%),number of oocysts shedding[(4.7-7.3)×10~5/g,(4.6-6.9)×10~5/g)]and survival time of mice(124-351 h.132-348 h)after inoculation(P>0.05).Conclusions Both the oocysts kept in liquid hifor longer preservation period of oocysts.