CAJ | 학술논문

目的探讨右美托咪啶对脂多糖(LPS)诱导大鼠外周血单核细胞Toll样受体4(TLR4)mRNA表达的影响.方法健康雄性Wistar大鼠40只,取外周血分离培养单核细胞,采用随机数字表法,将其随机分为5组(n=8),A组:阴性对照;B组:单核细胞中加入LPS(终浓度为1μg/ml);C组:单核细胞中加入LPS(终浓度为1μg/ml)+右美托咪啶(终浓度为0.5 ng/ml);D组:单核细胞中加入LPS(终浓度为1μg/ml)+右美托咪啶(终浓度为5.0 ng/ml);E组:单核细胞中加入LPS(终浓度为1μg/ml)+右美托咪啶(终浓度为50.0 ng/ml).孵育24 h后,收集上清液,采用ELISA法测定TNF-α、IL-1β和IL-6的浓度,采用RT-PCR法测定TLR4 mRNA的表达.结果与A组比较,B组TNF-α、IL-1p、IL-6的浓度升高,TLR4 mRNA表达上调(P＜0.01);与B组比较,C组、D组和E组TNF-α、IL-1β、IL-6的浓度降低,TLR4 mRNA表达下调(P＜0.05或0.01);与C组比较,D组和E组TNF-α、IL-1β、IL-6的浓度降低(P＜0.01),TLR4 mRNA表达差异无统计学意义(P＞0.05);D组和E组各指标比较差异无统计学意义(P＞0.05).结论右美托咪啶可通过下调TLR4 mRNA表达,抑制TLR4的合成,从而抑制LPS诱导大鼠外周血单核细胞TNF-α、IL-1β和IL-6的生成与释放.
목적 탐토우미탁미정대지다당(LPS)유도대서외주혈단핵세포Toll양수체4(TLR4)mRNA표체적영향.방법 건강웅성Wistar대서40지,취외주혈분리배양단핵세포,채용수궤수자표법,장기수궤분위5조(n=8),A조:음성대조;B조:단핵세포중가입LPS(종농도위1μg/ml);C조:단핵세포중가입LPS(종농도위1μg/ml)+우미탁미정(종농도위0.5 ng/ml);D조:단핵세포중가입LPS(종농도위1μg/ml)+우미탁미정(종농도위5.0 ng/ml);E조:단핵세포중가입LPS(종농도위1μg/ml)+우미탁미정(종농도위50.0 ng/ml).부육24 h후,수집상청액,채용ELISA법측정TNF-α、IL-1β화IL-6적농도,채용RT-PCR법측정TLR4 mRNA적표체.결과 여A조비교,B조TNF-α、IL-1p、IL-6적농도승고,TLR4 mRNA표체상조(P＜0.01);여B조비교,C조、D조화E조TNF-α、IL-1β、IL-6적농도강저,TLR4 mRNA표체하조(P＜0.05혹0.01);여C조비교,D조화E조TNF-α、IL-1β、IL-6적농도강저(P＜0.01),TLR4 mRNA표체차이무통계학의의(P＞0.05);D조화E조각지표비교차이무통계학의의(P＞0.05).결론 우미탁미정가통과하조TLR4 mRNA표체,억제TLR4적합성,종이억제LPS유도대서외주혈단핵세포TNF-α、IL-1β화IL-6적생성여석방.
Objective To investigate the effects of different concentrations of dexmedetomidine on the expression of Toll-like receptor 4 (TLR4) mRNA in rat peripheral blood monocytes exposed to lipopolysaccharide ( LPS ). Methods Peripheral blood monocytes isolated from male Wistar rats were seeded in 24-well plate in RPMI 1640 liquid culture medium in CO2 incubator at 37 ℃ and 5% CO2 for 2 h, and were randomly divided into 5 groups ( n = 8 each): group A negative control; group B was exposed to LPS 1 μg/ml and C, D and E groups were exposed to LPS 1 μg/ml + dexmetomidine 0.5, 5.0 and 50.0 ng/ml respectively. The monocytes were then incubated for 24 h. The concentrations of TNF-α, IL-1β and IL-6 in the supernatant of the cultured monocytes were detected by ELISA. The expression of TLR4 mRNA in the monocytes was detected by RT-PCR.Results Exposure to LPS significantly increased the expression of TLR4 mRNA and the concentrations of TNF-α, IL-1β and IL -6 in group B as compared with group A ( P ＜ 0.01 ). Dexmedetomidine attenuated the LPS-induced increase in the expression of TLR 4 mRNA and the concentrations of TNF-α, IL-1β and IL-6 in a dose-dependent manner ( P ＜0.05or 0.01 ). Conclusion Dexmedetomidine can inhibit the synthesis of TLR4 and inhibit the secretion and dilivery of TNF-α, IL-1β and IL-6 by down-regulating the gene expression of TLR4 in rat peripheral blood monocytes exposed to LPS.