中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
11期
1410-1411
,共2页
胡明政%吴建武%张伯%秦磊%钱海鑫%王学浩
鬍明政%吳建武%張伯%秦磊%錢海鑫%王學浩
호명정%오건무%장백%진뢰%전해흠%왕학호
肝%血红素氧合酶-l%缺血%再灌注损伤
肝%血紅素氧閤酶-l%缺血%再灌註損傷
간%혈홍소양합매-l%결혈%재관주손상
Liver%Heine oxgygenase-1%Ischemia%Reperfusian injury
目的 应用转染血红索氧合酶-1(HO-1)基因研究其在大鼠供肝缺血再灌损伤(IRI)中的抗凋亡作用.方法 将转染HO-1基因的腺病毒载体和空载体分别注入供体大鼠腹腔(n=8),36 h后取肝冷保存4 h行大鼠原位肝移植.缺血再灌注6 h检测肝功能、肝细胞凋亡率和肝组织HO-1、bcl-2、hel-xl和Caspase-3的表达.结果 (1)实验组的肝功能明显改善、肝细胞凋亡率显著低于对照组[(1.09±0.28)%比(8.30±1.08)%,P<0.01].(2)Western blot检测肝组织HO-1、bel-2和bcl-xl,灰度比值实验组显著高于对照组(HO-1:0.275±0.065比0.035±0.03;bcl-2:0.275±0.025比0.06±0.07;bcl-xl:(0.099±0.041比0.064±0.064,P<0.01),而Caspase-3则显著低于对照组(0.08±0.04比0.21±0.09,P<0.01).结论 HO-1在供肝IRI中通过上调bel-2、bel-xl和下调Caspase-3对供肝有显著的抗凋亡保护作用.
目的 應用轉染血紅索氧閤酶-1(HO-1)基因研究其在大鼠供肝缺血再灌損傷(IRI)中的抗凋亡作用.方法 將轉染HO-1基因的腺病毒載體和空載體分彆註入供體大鼠腹腔(n=8),36 h後取肝冷保存4 h行大鼠原位肝移植.缺血再灌註6 h檢測肝功能、肝細胞凋亡率和肝組織HO-1、bcl-2、hel-xl和Caspase-3的錶達.結果 (1)實驗組的肝功能明顯改善、肝細胞凋亡率顯著低于對照組[(1.09±0.28)%比(8.30±1.08)%,P<0.01].(2)Western blot檢測肝組織HO-1、bel-2和bcl-xl,灰度比值實驗組顯著高于對照組(HO-1:0.275±0.065比0.035±0.03;bcl-2:0.275±0.025比0.06±0.07;bcl-xl:(0.099±0.041比0.064±0.064,P<0.01),而Caspase-3則顯著低于對照組(0.08±0.04比0.21±0.09,P<0.01).結論 HO-1在供肝IRI中通過上調bel-2、bel-xl和下調Caspase-3對供肝有顯著的抗凋亡保護作用.
목적 응용전염혈홍색양합매-1(HO-1)기인연구기재대서공간결혈재관손상(IRI)중적항조망작용.방법 장전염HO-1기인적선병독재체화공재체분별주입공체대서복강(n=8),36 h후취간랭보존4 h행대서원위간이식.결혈재관주6 h검측간공능、간세포조망솔화간조직HO-1、bcl-2、hel-xl화Caspase-3적표체.결과 (1)실험조적간공능명현개선、간세포조망솔현저저우대조조[(1.09±0.28)%비(8.30±1.08)%,P<0.01].(2)Western blot검측간조직HO-1、bel-2화bcl-xl,회도비치실험조현저고우대조조(HO-1:0.275±0.065비0.035±0.03;bcl-2:0.275±0.025비0.06±0.07;bcl-xl:(0.099±0.041비0.064±0.064,P<0.01),이Caspase-3칙현저저우대조조(0.08±0.04비0.21±0.09,P<0.01).결론 HO-1재공간IRI중통과상조bel-2、bel-xl화하조Caspase-3대공간유현저적항조망보호작용.
Objective To investigate the antiapoptopic effect of heme oxgygenase-1 (HO-1) gene transfer in a rat model of IRI followed by orthotopic liver transplantation (OLT). Methods Control and experimental donors (n = 8) were intraperitoneally pretreated with Ad-EGFP and Ad-HO-1 respective-ly 36 h before their livers were harvested. Six h after reperfusion, serum ALT, AST and LDH levels were determined and the apoptotic ratio was analyzed by flow cytometer. The expression of HO-I, bel-2, bcl-xl and Caspase-3 was detected by Western-blot. Results (1) The expression level of HO-1 in the experi- mental group was significantly higher than in the control group (0.275±0.065 vs 0.035±0.03, P <0.01). The liver function index was meliorated and the apoptotic ratio of hepatic cells decreased signifi-cantly [(8.30±1.08) % vs (1.09±0.28) %] in the experimental group as compared with those in the control group; (2) The expression of bcl-2 and bcl-xl in the experimental group was increased significant-ly (bcl-2:0.06±0.07 vs 0.275±0.025;bcl-xl:0.064±0.064 vs 0.099±0.041 ,P <0.01) ,and the expression of casepase-3 was weakened obviously (0.21±0.09 vs 0.08±0.04, P < 0.01) as compared with those in the control group. Conclusion HO-1 can alleviate IRI in rat liver by suppressing apoptosis which relates to significantly modulating proapoptotic (Caspase-3) and anfiapoptotic (bcl-2 and bcl-xl) pathways.
