CAJ | 학술논문

目的研究不同病理类型和病理分期的非小细胞肺癌组织中SOX4基因序列突变情况,探讨SOX4基因在肺癌发生过程中的作用.方法 PCR扩增肺癌及癌旁组织SOX4基因HMG-box,并对经单链构象多态性分析有差异的20例病例进行测序.DNA及氨基酸序列比较利用Clustal和DNAStar软件.结果 90例肺癌组织标本中,有18例发生碱基突变.其中,鳞癌有7例,腺癌5例,腺鳞癌混合型6例,对照的癌旁组织中未检测出突变.临床Ⅱ、Ⅲ期的突变率明显高于Ⅰ期.结论 SOX4基因突变与肺癌肿瘤的病理类型无关,但与其病理分期有很大关系,提示SOX4基因突变可能与肺癌的发展和转移有某种关系,为探索SOX基因突变与人类肿瘤发生之间的关系提供了分子依据.
목적 연구불동병리류형화병리분기적비소세포폐암조직중SOX4기인서렬돌변정황,탐토SOX4기인재폐암발생과정중적작용.방법 PCR확증폐암급암방조직SOX4기인HMG-box,병대경단련구상다태성분석유차이적20례병례진행측서.DNA급안기산서렬비교이용Clustal화DNAStar연건.결과 90례폐암조직표본중,유18례발생감기돌변.기중,린암유7례,선암5례,선린암혼합형6례,대조적암방조직중미검측출돌변.림상Ⅱ、Ⅲ기적돌변솔명현고우Ⅰ기.결론 SOX4기인돌변여폐암종류적병리류형무관,단여기병리분기유흔대관계,제시SOX4기인돌변가능여폐암적발전화전이유모충관계,위탐색SOX기인돌변여인류종류발생지간적관계제공료분자의거.
Objective To investigate the mutation of SOX4 gene in the different tumor tissue with pathological stages and types of non-small cell lung cancer (NSCLC), and to explore its roles in the progression of lung carcinoma.Methods The SOX4 gene HMG-box of lung cancer tissues and paracancerous tissues were amplified by PCR, 20 cases shown difference by single strand conformation polymorphyism analysis were sequenced. The DNA sequences were compared with normal sequences by software Clustal and DNAStar. Results In the 90 NSCLCs, 18 cases were found with mutations of SOX4 gene and were sequenced, and there were 2 mutational points. Seven were detected from squamous cell carcinoma, five from adenocarcinoma and six from adeno-squamous. Three were obtained from tissues in stage Ⅰ ,five in stage Ⅱ , six in stage Ⅲ, and four in stage Ⅳ. The mutation rate in stage Ⅱ , Ⅲ and Ⅳ was significantly higher than that in stage Ⅰ . Conclusion SOX4 gene mutation is not associated with pathology histological types of tumor, but it is significantly associated with pathological stages and the mutation rate increases gradually, which has relation with advanced pathological stages in NSCLC. The results indicate that the SOX4 gene mutations might be related in the lung carcinogenesis and tumor metastasis. The study also provides molecular data for study the links between the mutation of SOX gene and human oncogenesis.