CAJ | 학술논문

以pLAFR3为载体构建重组质粒pHN207，携带有来自苜蓿根瘤菌（Sinorhizobium meliloti）的四碳二羧酸转移酶基因dctABD、来自pTR102的parCBA／DE基因和标记发光酶基因luxAB。利用2亲本杂交法，将重组质粒pHN207导入大豆慢生根瘤菌（Bradyrhizobium japonicum）TA11和CB1809，分别考察了转移接合子中外源重组质粒在人工培养条件和共生条件下的稳定性，结果表明par基因的引入明显提高pLAFR3在TA11和CB1809中的稳定性。dctABD基因可显著提高TA11和CB1809在大豆黑龙33、宁镇一号和渝豆一号上的共生固氮能力，使结瘤植物的地上部分干重（生物量）和总氮量等指标较对照组有显著提高。
이pLAFR3위재체구건중조질립pHN207，휴대유래자목숙근류균（Sinorhizobium meliloti）적사탄이최산전이매기인dctABD、래자pTR102적parCBA／DE기인화표기발광매기인luxAB。이용2친본잡교법，장중조질립pHN207도입대두만생근류균（Bradyrhizobium japonicum）TA11화CB1809，분별고찰료전이접합자중외원중조질립재인공배양조건화공생조건하적은정성，결과표명par기인적인입명현제고pLAFR3재TA11화CB1809중적은정성。dctABD기인가현저제고TA11화CB1809재대두흑룡33、저진일호화투두일호상적공생고담능력，사결류식물적지상부분간중（생물량）화총담량등지표교대조조유현저제고。
ArecombinantplasmidpHN207containingC4-dicarboxylicacidtransport genes(dctABD) from Sinorhizobium meliloti, parCBA／DE genes from pTR102 and reporter genes luxAB from pDB30 was constructed by using pLAFR3 as the vector. The pHN207 was then introduced into the Bradyrhizobium japonicum TA11 and CB1809 by bi-parental mating. It was confirmed that parCBA／DE genes could increase the stability of pLAFR3 in the transconjugants under both free-living and symbiotic condition. The results of plant pot experiment indicated that the introduction of dctABD genes could significantly improve the symbiotic nitrogen fixation efficiency of TA11 and CB1809 with soybean varieties of Heilong 33, Ningzhen No.1 and Yudou No.1. Compared with the control, the shoot dry weight (biomass) and total nitrogen content of the plants tested were significantly increased.