浙江大学学报(英文版)
浙江大學學報(英文版)
절강대학학보(영문판)
JOURNAL OF ZHEJIANG UNIVERSITY SCIENCE
2004年
10期
1199-1205
,共7页
严成其%钱凯先%薛刚平%吴忠长%陈跃磊%颜秋生%张雪琴%吴平
嚴成其%錢凱先%薛剛平%吳忠長%陳躍磊%顏鞦生%張雪琴%吳平
엄성기%전개선%설강평%오충장%진약뢰%안추생%장설금%오평
Oryza sativa L.%Oryza meyeriana L.%Somatic hybridization%Rice bacterial blight resistance
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance.